DAPK3 Protein (GST tag)
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- Target See all DAPK3 Proteins
- DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This DAPK3 protein is labelled with GST tag.
- Purpose
- Recombinant Human DAPK3/ZIPK Protein (GST Tag)(Active)
- Sequence
- Met 1-Arg 454
- Characteristics
- A DNA sequence encoding the full length of human DAPK3 (NP_001339.1) (Met 1-Arg 454) was fused with the GST tag at the N-terminus.
- Purity
- > 85 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 5 nmol/min/mg using MBP as substrate.
- Top Product
- Discover our top product DAPK3 Protein
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Death-Associated Protein Kinase 3 (DAPK3) (AA 1-454) (Active) protein (GST tag)
DAPK3 Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
Death-Associated Protein Kinase 3 (DAPK3) (AA 1-454) protein (GST tag)DAPK3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 3 (DAPK3) (AA 355-454) protein (GST tag)DAPK3 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Death-Associated Protein Kinase 3 (DAPK3) (AA 1-454) protein (Strep Tag)Crystallography grade DAPK3 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Death-Associated Protein Kinase 3 (DAPK3) (AA 1-448) protein (Strep Tag)Crystallography grade DAPK3 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Death-Associated Protein Kinase 3 (DAPK3) protein (Myc-DYKDDDDK Tag)DAPK3 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 mM GSH, pH 7.4
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- DAPK3 (Death-Associated Protein Kinase 3 (DAPK3))
- Alternative Name
- DAPK3/ZIPK (DAPK3 Products)
- Synonyms
- DAPK3 Protein, dapk3 Protein, wu:fc25b08 Protein, si:dkey-57k2.5 Protein, MGC114871 Protein, ZIP Protein, ZIPK Protein, dlk Protein, Dapkl Protein, death associated protein kinase 3 Protein, death-associated protein kinase 3 Protein, death-associated protein kinase 3 L homeolog Protein, DAPK3 Protein, dapk3 Protein, dapk3.L Protein, Dapk3 Protein
- Background
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Background: Death-associated protein kinase 3, also known as DAP kinase 3, ZIP-kinase, DAPK3 and ZIPK, is a nucleus and cytoplasm protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and DAP kinase subfamily. DAPK3 / ZIPK contains one protein kinase domain. It is a serine/threonine kinase which acts as a positive regulator of apoptosis. It phosphorylates histone H3 on 'Thr-11' at centromeres during mitosis. DAPK3 / ZIPK is a homodimer or forms heterodimers with ATF4. Both interactions require an intact leucine zipper domain and oligomerization is required for full enzymatic activity. It also binds to DAXX and PAWR, possibly in a ternary complex which plays a role in caspase activation. DAPK3 / ZIPK regulates myosin light chain phosphatase through phosphorylation of MYPT1 thereby regulating the assembly of the actin cytoskeleton, cell migration, invasiveness of tumor cells, smooth muscle contraction and neurite outgrowth. It is involved in the formation of promyelocytic leukemia protein nuclear body (PML-NB), one of many subnuclear domains in the eukaryotic cell nucleus, and which is involved in oncogenesis and viral infection.
Synonym: DLK;ZIP;ZIPK
- Molecular Weight
- 79 kDa
- NCBI Accession
- NP_001339
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