DCLK1 Protein (AA 1-705) (GST tag,His tag)

Catalog No. ABIN7317068

Product Details

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Protein Type: Recombinant

Biological Activity: Active

Protein Characteristics: AA 1-705

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This DCLK1 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human DCAMKL1 Protein (aa 1-705, His & GST Tag)(Active)

Sequence: Met 1-Val 705

Characteristics: A DNA sequence encoding the human DCLK1 (O15075-1) (Met 1-Val 705) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 84 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 6.1 nmol/min/mg using synthetic Autocamtide-2 peptide (KKALRRQETVDAL-amide) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 0.5 mM PMSF

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: DCLK1 (Doublecortin-Like Kinase 1 (DCLK1))

Alternative Name: DCAMKL1 (DCLK1 Products)

Synonyms: DCLK1 Protein, DCAMKL1 Protein, dcamkl1 Protein, dclk1 Protein, wu:fc51f11 Protein, zgc:153709 Protein, MGC145348 Protein, CL1 Protein, CLICK1 Protein, DCDC3A Protein, DCLK Protein, 1700113D08Rik Protein, 2810480F11Rik Protein, AI836758 Protein, Click-I Protein, Cpg16 Protein, Dcamkl1 Protein, Dcl Protein, Dclk Protein, mKIAA0369 Protein, Ania4 Protein, doublecortin like kinase 1 Protein, doublecortin-like kinase 1a Protein, serine/threonine-protein kinase DCLK1 Protein, doublecortin-like kinase 2 Protein, doublecortin-like kinase 1 Protein, DCLK1 Protein, dclk1a Protein, LOC587664 Protein, dclk2 Protein, LOC100539645 Protein, Dclk1 Protein

Background:

Background: DCAMKL1, also known as DCLK1, is a member of the protein kinase superfamily and the doublecortin family. It contains two N-terminal doublecortin domains, which bind microtubules and regulate microtubule polymerization, a C-terminal serine/threonine protein kinase domain, which shows substantial homology to Ca2+/calmodulin-dependent protein kinase, and a serine/proline-rich domain in between the doublecortin and the protein kinase domains, which mediates multiple protein-protein interactions. DCAMKL1 is involved in several different cellular processes, including neuronal migration, retrograde transport, neuronal apoptosis and neurogenesis. Its microtubule-polymerizing activity is independent of its protein kinase activity. DCAMKL1 may be involved in a calcium-signaling pathway controlling neuronal migration in the developing brain. It may also participate in functions of the mature nervous system.

Synonym: CL1,CLICK1,DCAMKL1,DCDC3A,DCLK

Molecular Weight: 106 kDa