DYRK3 Protein (GST tag,His tag)

Catalog No. ABIN7317092

Product Details

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This DYRK3 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human DYRK3/REDK Protein (His & GST Tag)(Active)

Sequence: Met 1-Ser 588

Characteristics: A DNA sequence encoding the human DYRK3 isoform 1 (O43781-1) (Met 1-Ser 588) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 85 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 22 nmol/min/mg using synthetic DYRKtide peptide (RRRFRPASPLRGPPK) as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 20 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.0

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: DYRK3 (Dual-Specificity tyrosine-(Y)-phosphorylation Regulated Kinase 3 (DYRK3))

Alternative Name: DYRK3/REDK (DYRK3 Products)

Synonyms: DYRK5 Protein, RED Protein, REDK Protein, hYAK3-2 Protein, BC006704 Protein, DYRK3 Protein, MGC89944 Protein, dual specificity tyrosine phosphorylation regulated kinase 3 Protein, dual-specificity tyrosine-(Y)-phosphorylation regulated kinase 3 Protein, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 3 Protein, dual specificity tyrosine-(Y)-phosphorylation regulated kinase 3 L homeolog Protein, DYRK3 Protein, Dyrk3 Protein, dyrk3 Protein, dyrk3.L Protein

Background:

Background: Dual specificity tyrosine-phosphorylation-regulated kinase 3, also known as Regulatory erythroid kinase, REDK and DYRK3, is a nucleus protein which belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MNB/DYRK subfamily. DYRKs are an emerging family of dual-specificity kinases that play key roles in cell proliferation, survival, and development. DYRK3 contains one protein kinase domain. Isoform 1 and isoform 2 of DYRK3 are highly expressed in testis and in hematopoietic tissue such as fetal liver, and bone marrow. Isoform 2 of DYRK3 is the predominant form in testis. Isoform 1 of DYRK3 is the predominant form in fetal liver and bone marrow. Isoform 1 and isoform 2 are present at low levels in heart, pancreas, lymph node, and thymus. DYRK3 is a negative regulator of EPO-dependent erythropoiesis. It may place an upper limit on red cell production during stress erythropoiesis. DYRK3 inhibits cell death due to cytokine withdrawal in hematopoietic progenitor cells. It may also act by regulating CREB/CRE signaling. DYRK3 proved to effectively inhibit NFAT (nuclear factor of activated T cells) transcriptional response pathways and to co-immunoprecipitate with NFATc3. DYRK3 attenuates (and possibly apportions) red cell production selectively during anemia.

Synonym: DYRK5;hYAK3-2;RED;REDK

Molecular Weight: 93.5 kDa

Pathways: Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha