ERN1 Protein (AA 465-977) (GST tag,His tag)
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- Target See all ERN1 Proteins
- ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))
- Protein Type
- Recombinant
- Biological Activity
- Active
- Protein Characteristics
- AA 465-977
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This ERN1 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human ERN1/IRE1 Protein (aa 465-977, His & GST Tag)(Active)
- Sequence
- Pro 465-Leu 977
- Characteristics
- A DNA sequence encoding the human ERN1 isoform 1 (O75460-1) cytoplasmic domain (Pro 465-Leu 977) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 80 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- 1.Kinase activity untested 2. The specific activity was determined to be 5 nmol/min/mg using MBP as substrate
- Top Product
- Discover our top product ERN1 Protein
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Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 1-70) protein (GST tag)
ERN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 401-500) protein (GST tag)ERN1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 19-443) protein (His tag)Crystallography grade ERN1 Origin: Human Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 21-445) protein (His tag)Crystallography grade ERN1 Origin: Mouse Host: Escherichia coli (E. coli) Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 465-977) protein (His tag)Crystallography grade ERN1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 467-977) protein (His tag)Crystallography grade ERN1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 19-977) protein (rho-1D4 tag)Crystallography grade ERN1 Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) (AA 21-977) protein (rho-1D4 tag)Crystallography grade ERN1 Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1) protein (Myc-DYKDDDDK Tag)ERN1 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 7.4
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))
- Alternative Name
- ERN1/IRE1 (ERN1 Products)
- Synonyms
- IRE1 Protein, IRE1P Protein, IRE1a Protein, hIRE1p Protein, 9030414B18Rik Protein, AI225830 Protein, C85377 Protein, Ire1a Protein, Ire1alpha Protein, Ire1p Protein, RGD1559716 Protein, Ern1 Protein, Ire1 Protein, Ire1b Protein, sb:eu1005 Protein, zgc:109932 Protein, endoplasmic reticulum to nucleus signaling 1 Protein, endoplasmic reticulum to nucleus signaling 2 Protein, endoplasmic reticulum (ER) to nucleus signalling 1 Protein, serine/threonine-protein kinase/endoribonuclease IRE1 Protein, endoplasmic reticulum (ER) to nucleus signalling 2 Protein, ERN1 Protein, ERN2 Protein, Ern1 Protein, LOC524719 Protein, Ern2 Protein, ern1 Protein
- Background
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Background: The encoded protein contains two functional catalytic domains, a serine/threonine-protein kinase domain and an endoribonuclease domain. This protein functions as a sensor of unfolded proteins in the endoplasmic reticulum (ER) and triggers an intracellular signaling pathway termed the unfolded protein response (UPR). The UPR is an ER stress response that is conserved from yeast to mammals and activates genes involved in degrading misfolded proteins, regulating protein synthesis and activating molecular chaperones. This protein specifically mediates the splicing and activation of the stress response transcription factor X-box binding protein 1.
Synonym: hIRE1p,IRE1,IRE1a,IRE1P
- Molecular Weight
- 86 kDa
- Pathways
- ER-Nucleus Signaling, Unfolded Protein Response
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