ECE2 Protein (His tag)

Catalog No. ABIN7195505

Product Details

Target: ECE2 (Endothelin Converting Enzyme 2 (ECE2))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ECE2 protein is labelled with His tag.

Purpose: Recombinant Human ECE-2 Protein (His Tag)

Sequence: Gly 199-Trp 883

Characteristics: A DNA sequence encoding the ectodomain of human endothelin converting enzyme 2 isoform A (NP_055508.3) (Gly 199-Trp 883) was fused with a polyhistidine tag at the N-terminus.

Purity: > 93 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ECE2 (Endothelin Converting Enzyme 2 (ECE2))

Alternative Name: ECE-2 (ECE2 Products)

Synonyms: ECE2 Protein, 1810009K13Rik Protein, 6330509A19Rik Protein, 9630025D12Rik Protein, BB127715 Protein, endothelin converting enzyme 2 Protein, endothelin-converting enzyme 2 Protein, ECE2 Protein, LOC702851 Protein, ece2 Protein, LOC100543478 Protein, Ece2 Protein

Background:

Background: Endothelin-converting enzyme 2, also known as ECE-2, is a metalloprotease that possesses many properties consistent with it being a neuropeptide-processing enzyme. Endothelin-converting enzymes (ECEs) are the key enzymes in the endothelin (ET) biosynthesis that catalyze the conversion of big ET, the biologically inactive precursor of mature ET. Two enzymes, termed ECE-1 and ECE-2, have been molecularly identified. ECE-2 is found primarily in neural tissues, with high levels of expression in midbrain, cerebellum, hypothalamus, frontal cortex and spinal cord and moderate levels in hippocampus and striatum. ECE-2 is strongly down-regulated in inferior parietal lobe from Alzheimer disease patients (at protein level). ECE-2 converts big endothelin-1 to endothelin-1. It is involved in the processing of various neuroendocrine peptides, including neurotensin, angiotensin I, substance P, proenkephalin-derived peptides, and prodynorphin-derived peptides. ECE-2 may limit beta-amyloid peptide accumulation in brain. It may also have methyltransferase activity. A comparison of residues around the cleavage site revealed that ECE-2 exhibits a unique cleavage site selectivity that is related to but distinct from that of ECE-1.

Synonym: ECE2,hCG_2022032,KIAA0604,MGC17664,MGC2408,MGC78487

Molecular Weight: 80.2 kDa

NCBI Accession: NP_055508

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling