RNASE3 Protein (His tag)

Catalog No. ABIN7318990

Product Details

Target: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Protein Type: Recombinant

Origin: Human

Source: Human Cells

Purification tag / Conjugate: This RNASE3 protein is labelled with His tag.

Purpose: Recombinant Human Ribonuclease 3/RNASE3 Protein (His Tag)

Sequence: Arg28-Ile160

Characteristics: Recombinant Human Ribonuclease 3 is produced by our Mammalian expression system and the target gene encoding Arg28-Ile160 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as a 0.2 μm filtered solution of 20 mM TrisHCl,150 mM NaCl,1 mMDTT,10 % Glycerol, pH 7.5.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: RNASE3 (ECP) (Eosinophil Cationic Protein (ECP))

Alternative Name: Ribonuclease 3/RNASE3 (ECP Products)

Synonyms: ECP Protein, RNS3 Protein, ribonuclease A family member 3 Protein, RNASE3 Protein

Background:

Background: Ribonuclease 3 (RNASE3) is a basic protein that is localized to the eosinophil primary matrix and belongs to the pancreatic ribonuclease family. RNASE3 is released during degranulation of eosinophils. RNASE3 possesses a wide variety of biological activities. RNASE3 interacts with bacterial lipopolysaccharide (LPS) and lipoteichoic acid (LTA). RNASE3 exhibits antibacterial activity, including cytoplasmic membrane depolarization of preferentially Gram-negative, but also Gram-positive strains. It promotes E. coli outer membrane detachment, alteration of the overall cell shape and partial loss of cell content.

Synonym: Eosinophil Cationic Protein, ECP, Ribonuclease 3, RNase 3, RNASE3, ECP, RNS3

Molecular Weight: 16.6 kDa

UniProt: P12724