EPH Receptor B4 Protein (EPHB4) (AA 563-987) (His-GST)

Catalog No. ABIN7317112

This Recombinant EPH Receptor B4 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for EPH Receptor B4 Protein (EPHB4) (AA 563-987) (His-GST) (ABIN7317112)

Target: EPH Receptor B4 (EPHB4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 563-987

Purification tag / Conjugate: This EPH Receptor B4 protein is labelled with His-GST.

Purpose: Recombinant Human EphB4/HTK Protein (aa 563-987, His & GST Tag)

Sequence: Leu563-Tyr987

Characteristics: A DNA sequence encoding the human EPHB4 (P54760) (Leu563-Tyr987) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 47 nmol/min/mg using Poly(Glu:Tyr) 4:1 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 3 mM DTT, 10 % glycerol

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EphB4/HTK

Background: HTK,MYK1,TYRO11,Ephrin type-B receptor 4 is a protein that in humans is encoded by the EPHB4 gene. It is a single-pass type I membrane protein belonging to the ephrin receptor subfamily of protein kinase superfamily. Members of the ephrin and Eph family are local mediators of cell function through largely contact-dependent processes in development and in maturity. Furthermore, EphB4 protein and the corresponding ligand Ephrin-B2 contribute to tumor growth in various human tumors. EphB4 protein has tumor suppressor activities and that regulation of cell proliferation, extracellular matrix remodeling, and invasive potential are important mechanisms of tumor suppression. Therefore, Ephrin-B2/EphB4 may be recognized as a novel prognostic indicator for cancers.

Molecular Weight:

Calculated MW: 75.2 kDa

Observed MW: 66 kDa

Gene ID: 2050

UniProt: P54760

Pathways: RTK Signaling