EPH Receptor B4 Protein (EPHB4) (Fc Tag,His tag)

Catalog No. ABIN7535001

Product Details

Target: EPH Receptor B4 (EPHB4)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This EPH Receptor B4 protein is labelled with Fc Tag,His tag.

Purpose: Active Recombinant Human EphB4/HTK Protein

Sequence: MELRVLLCWA SLAAALEETL LNTKLETADL KWVTFPQVDG QWEELSGLDE EQHSVRTYEV CDVQRAPGQA HWLRTGWVPR RGAVHVYATL RFTMLECLSL PRAGRSCKET FTVFYYESDA DTATALTPAW MENPYIKVDT VAAEHLTRKR PGAEATGKVN VKTLRLGPLS KAGFYLAFQD QGACMALLSL HLFYKKCAQL TVNLTRFPET VPRELVVPVA GSCVVDAVPA PGPSPSLYCR EDGQWAEQPV TGCSCAPGFE AAEGNTKCRA CAQGTFKPLS GEGSCQPCPA NSHSNTIGSA VCQCRVGYFR ARTDPRGAPC TTPPSAPRSV VSRLNGSSLH LEWSAPLESG GREDLTYALR CRECRPGGSC APCGGDLTFD PGPRDLVEPW VVVRGLRPDF TYTFEVTALN GVSSLATGPV PFEPVNVTTD REVPPAVSDI RVTRSSPSSL SLAWAVPRAP SGAVLDYEVK YHEKGAEGPS SVRFLKTSEN RAELRGLKRG ASYLVQVRAR SEAGYGPFGQ EHHSQTQLDE SEGWREQLA

Specificity: Met1-Ala539

Purity: > 97 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: < 0.1 EU/μg of the protein by LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA. Immobilized Human EFNB2 at 0.5 μg/mL (100 μL/well) can bind Human EPHB4 with a linear range of 6-400 pg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80 °C for long term.
After reconstitution, the protein solution is stable at -20 °C for 3 months, at 2-8 °C for up to 1 week.

Target Details

Target: EPH Receptor B4 (EPHB4)

Alternative Name: EphB4/HTK (EPHB4 Products)

Synonyms: HTK Protein, MYK1 Protein, TYRO11 Protein, htk Protein, myk1 Protein, ephB4a Protein, tyro11 Protein, ephb4b Protein, MGC130997 Protein, AI042935 Protein, Htk Protein, MDK2 Protein, Myk1 Protein, Tyro11 Protein, cb646 Protein, ephB4 Protein, rtk5 Protein, EphB-rtk8 Protein, cb417 Protein, rtk8 Protein, EPH receptor B4 Protein, EPH receptor B4 L homeolog Protein, EPH receptor B4 S homeolog Protein, Eph receptor B4 Protein, eph receptor B4a Protein, eph receptor B4b Protein, EPHB4 Protein, ephb4.L Protein, ephb4.S Protein, ephb4 Protein, Ephb4 Protein, ephb4a Protein, ephb4b Protein

Background:

Description: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The protein encoded by this gene binds to ephrin-B2 and plays an essential role in vascular development.

Name: EPHB4,HFASD,HTK,MYK1,TYRO11

Gene ID: 2050

UniProt: P54760

Pathways: RTK Signaling