EGFR Protein (His tag)

Catalog No. ABIN7320051

This Recombinant EGFR protein is produced in HEK-293 Cells.

Quick Overview for EGFR Protein (His tag) (ABIN7320051)

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purity: > 95 % as determined by SDS-PAGE

Product Details

Purification tag / Conjugate: This EGFR protein is labelled with His tag.

Purpose: Recombinant Mouse EGFR Protein (His Tag)(Active)

Sequence: Met 1-Ser 647

Characteristics: A DNA sequence encoding the extracellular domain of mouse EGFR (Q01279) (Met 1-Ser 647) was fused with a polyhistidine tag at the C-terminus.

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  human EGF-Fc , The EC50 of human EGF-Fc is 60-90 ng/mL.3. Immobilized mouse EGFR-his at 10 μg/mL (100 μl/well) can bind  mouse EGF-Fc, The EC50 of mouse EGF-Fc is 70-100 ng/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: EGFR (Epidermal Growth Factor Receptor (EGFR))

Alternative Name: EGFR

Background:

Background: As a member of the epidermal growth factor receptor (EGFR) family, EGFR protein is type I transmembrane glycoprotein that binds a subset of EGF family ligands including EGF, amphiregulin, TGF-α, betacellulin, etc. EGFR protein plays a crucial role in signaling pathway in the regulation of cell proliferation, survival and differentiation. Binding of a ligand induces EGFR protein homo- or heterodimerization, the subsequent tyrosine autophosphorylation and initiates various down stream pathways (MAPK, PI3K/PKB and STAT). In addition, EGFR signaling also has been shown to exert action on carcinogenesis and disease progression, and thus EGFR protein is proposed as a target for cancer therapy currently.Immune Checkpoint   Immunotherapy   Cancer Immunotherapy   Targeted Therapy

Synonym: 9030024J15Rik;AI552599;Erbb;Errb1;Errp;wa-2;wa2;Wa5

Molecular Weight: 71 kDa

UniProt: Q01279

Pathways: NF-kappaB Signaling, RTK Signaling, Fc-epsilon Receptor Signaling Pathway, EGFR Signaling Pathway, Neurotrophin Signaling Pathway, Stem Cell Maintenance, Hepatitis C, Positive Regulation of Response to DNA Damage Stimulus, Interaction of EGFR with phospholipase C-gamma, Thromboxane A2 Receptor Signaling, EGFR Downregulation, S100 Proteins