FLRT1 Protein (His tag)
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- Target See all FLRT1 Proteins
- FLRT1 (Fibronectin Leucine Rich Transmembrane Protein 1 (FLRT1))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This FLRT1 protein is labelled with His tag.
- Purpose
- Recombinant Human FLRT1 Protein (His Tag)(Active)
- Sequence
- Met 1-Pro 524
- Characteristics
- A DNA sequence encoding the human FLRT1 extracellular domain (Q9NZU1-1) (Met 1-Pro 524) was expressed, fused with a polyhistidine tag at the C-terminus.
- Purity
- > 96 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When cells are added to coated plates(5μg/mL, 100μL/well), approximately 50%-70% will adhere after 1 hour at 37°C.
- Top Product
- Discover our top product FLRT1 Protein
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Fibronectin Leucine Rich Transmembrane Protein 1 (FLRT1) (AA 1-674) protein (GST tag)
FLRT1 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- FLRT1 (Fibronectin Leucine Rich Transmembrane Protein 1 (FLRT1))
- Alternative Name
- FLRT1 (FLRT1 Products)
- Synonyms
- MGC84476 Protein, FLRT1 Protein, sc:d0318 Protein, AW742165 Protein, D630040I23Rik Protein, RGD1565152 Protein, fibronectin leucine rich transmembrane protein 1 S homeolog Protein, fibronectin leucine rich transmembrane protein 1 Protein, fibronectin leucine rich transmembrane protein 1b Protein, flrt1.S Protein, FLRT1 Protein, flrt1b Protein, flrt1 Protein, Flrt1 Protein
- Background
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Background: The three fibronectin leucine-rich repeat transmembrane (FLRT) proteins contain 10 leucine-rich repeats (LRR), a type III fibronectin (FN) domain, followed by the transmembrane region, and a short cytoplasmic tail. FLRT1 is expressed in kidney and brain, which is a target for tyrosine phosphorylation mediated by FGFR1 and implicate a non-receptor Src family kinase (SFK). All FLRTs can interact with FGFR1 and FLRTs can be induced by the activation of FGF signalling by FGF-2. The phosphorylation state of FLRT1, which is itself FGFR1 dependent, may play a critical role in the potentiation of FGFR1 signalling and may also depend on a SFK-dependent phosphorylation mechanism acting via the FGFR. This is consistent with an 'in vivo' role for FLRT1 regulation of FGF signalling via SFKs. Furthermore, the phosphorylation-dependent futile cycle mechanism controlling FGFR1 signalling is concurrently crucial for regulation of FLRT1-mediated neurite outgrowth. FLRT1, FLRT2 and FLRT3 are members of the fibronectin leucine rich transmembrane protein (FLRT) family. They may function in cell adhesion and/or receptor signalling. Their protein structures resemble small leucine-rich proteoglycans found in the extracellular matrix. FLRT3 shares 55 % amino acid sequence identity with FLRT1.
Synonym: Leucine-Rich Repeat Transmembrane Protein FLRT1, Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 1, FLRT1
- Molecular Weight
- 57 kDa
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