FLRT3 Protein (His tag)

Catalog No. ABIN7317635

Product Details

Target: FLRT3 (Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This FLRT3 protein is labelled with His tag.

Purpose: Recombinant Human FLRT3 Protein (His Tag)(Active)

Sequence: Met 1-Pro 528

Characteristics: A DNA sequence encoding the human FLRT3 (NP_938205.1) extracellular domain (Met 1-Pro 528) was expressed, fused with a polyhistidine tag at the C-terminus.

Purity: > 98 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by the ability of the immobilized protein to support the adhesion of Neuro-2A mouse neuroblastoma cells.When cells are added to coated plates(5μg/mL, 100μL/well), approximately 50%-70% will adhere after 1 hour at 37°C.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FLRT3 (Fibronectin Leucine Rich Transmembrane Protein 3 (FLRT3))

Alternative Name: FLRT3 (FLRT3 Products)

Synonyms: flrt3 Protein, FLRT3 Protein, xflrt3 Protein, DKFZp459F141 Protein, 5530600M07Rik Protein, C430047I10Rik Protein, mKIAA1469 Protein, RGD1566005 Protein, HH21 Protein, fibronectin leucine rich transmembrane protein 3 S homeolog Protein, fibronectin leucine rich transmembrane protein 3 Protein, fibronectin leucine rich transmembrane protein 3 L homeolog Protein, flrt3.S Protein, FLRT3 Protein, flrt3.L Protein, flrt3 Protein, Flrt3 Protein

Background:

Background: Leucine-rich repeat transmembrane protein FLRT3, also known as Fibronectin-like domain-containing leucine-rich transmembrane protein 3, and FLRT3, is a single-pass type I membrane protein which belongs to the fibronectin leucine rich transmembrane protein (FLRT) family. FLRT3 contains one fibronectin type-III domain and ten LRR (leucine-rich) repeats and is expressed in kidney, brain, pancreas, skeletal muscle, lung, liver, placenta, and heart. It has a provocative expression pattern during somite development being expressed in regions of the somite where muscle precursor cells migrate from the dermomyotome and move into the myotome, and later in myotomal precursors destined to migrate towards their final destination. FLRT1, FLRT2 and FLRT3 are members of the FLRT family. The FLRT family of leucine-rich repeat (LRR) proteins is implicated in fibroblast growth factor (FGF) signalling, early embryonic development and neurite outgrowth. FLRT3 shares 55 % amino acid sequence identity with FLRT1 and 44 % identity with FLRT2. Two alternatively spliced transcript variants encoding the same protein have been described. The expression of FLRT3 is controlled by fibroblast growth factors (FGFs). FLRT3 has been implicated in neurite outgrowth after nerve damage, as a positive regulator of FGF signalling and in homotypic cell adhesion. FLRT3 may have a crucial role in regulating cellular adhesion between the epithelial apical ridge and the underlying mesenchyme and in establishing the dorso-ventral position of the ridge.

Synonym: Leucine-Rich Repeat Transmembrane Protein FLRT3, Fibronectin-Like Domain-Containing Leucine-Rich Transmembrane Protein 3, FLRT3, KIAA1469,HH21

Molecular Weight: 58 kDa

NCBI Accession: NP_938205