FSTL3 Protein (His tag)

Catalog No. ABIN7320428

Product Details

Target: FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This FSTL3 protein is labelled with His tag.

Purpose: Recombinant Mouse FLRG/Fstl3 Protein (His Tag)(Active)

Sequence: Met 1-Val 256

Characteristics: A DNA sequence encoding the mouse FLRG (NP_113557.1) (Met 1-Val 256) was fused with a polyhistidine tag at the C-terminus.

Purity: > 85 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA. Immobilized mouse FLRG-His at 10 μg/ml (100 μl/well) can bind biotinylated human INHBA-His with a linear range of 6.25-50 ng/ml.2. Measured by its binding ability in a functional ELISA. Immobilized mouse FLRG-His at 10 μg/ml (100 μl/well) can bind biotinylated mouse INHBA-His with a linear range of 6.25-50 ng/ml.3. Measured by its ability to neutralize Activin-mediated inhibition on MPC11 cell proliferation.The ED50 for this effect is typically 5-25 ng/mL in the presence of 10 ng/mL rhActivin A.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: FSTL3 (Follistatin-Like 3 (Secreted Glycoprotein) (FSTL3))

Alternative Name: FLRG/Fstl3 (FSTL3 Products)

Synonyms: E030038F23Rik Protein, Flrg Protein, FLRG Protein, FSRP Protein, follistatin-like 3 Protein, follistatin like 3 Protein, Fstl3 Protein, FSTL3 Protein

Background:

Background: Follistatin-like 3 (FLRG/Fstl3) is a secreted glycoprotein of the follistatin-module-protein family. It may have a role in leukemogenesis. FLRG/Fstl3 is a recently described member of the FST family having an overall structure and activity profile similar to that of FST, including binding and neutralization of activin. FLRG/Fstl3 is expressed in a wide range of adult tissues, not detected in hematopoietic cells except in patients with a B cell chronic leukemia and a translocation. Isoform 1 or the secreted form is a binding and antagonizing protein for members of the TGF-beta family, such us activin, BMP2 and MSTN. Inhibits activin A-, activin B-, BMP2- and MSDT-induced cellular signaling; more effective on activin A than on activin B. Involved in bone formation; inhibits osteoclast differentiationc. Involved in hematopoiesis; involved in differentiation of hemopoietic progenitor cells, increases hematopoietic cell adhesion to fibronectin and seems to contribute to the adhesion of hematopoietic precursor cells to the bone marrow stroma. Isoform 2 of FLRG/Fstl3 or the nuclear form of FLRG/Fstl3 is probably involved in transcriptional regulation via interaction with MLLT10. Modulation of activin and other TGFβ superfamily signaling is the primary mechanism of action for both follistatin (FS) and FS-like 3 (FSTL-3). FLRG/Fstl3 is likely to be a local regulator of activin action in gonadal development and gametogenesis and, further, that activin appears to have important actions in gonadal development and function that are critical for normal reproduction.

Synonym: E030038F23Rik;Flrg

Molecular Weight: 26 kDa

NCBI Accession: NP_113557