GNGT1 Protein (His tag)

Catalog No. ABIN7317214

Product Details

Target: GNGT1 (G Protein gamma Transducing Activity Polypeptide 1 (GNGT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This GNGT1 protein is labelled with His tag.

Purpose: Recombinant Human GNGT1/GNG1 Protein (His Tag)

Sequence: Pro 2-Cys 71

Characteristics: A DNA sequence encoding the mature foem of human GNGT1 (P63211) (Pro 2-Cys 71) was expressed, with a polyhistide tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.5

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: GNGT1 (G Protein gamma Transducing Activity Polypeptide 1 (GNGT1))

Alternative Name: GNGT1/GNG1 (GNGT1 Products)

Synonyms: GNG1 Protein, G(y)1 Protein, Gng1 Protein, G protein subunit gamma transducin 1 Protein, guanine nucleotide binding protein (G protein), gamma transducing activity polypeptide 1 Protein, GNGT1 Protein, Gngt1 Protein

Background:

Background: GNGT1 is a subunit of of transducin. Heterotrimeric G proteins consist of alpha, beta, and gamma subunits. They are membrane bound GTPases that are linked to 7-TM receptors. They function as signal transducers for the 7-transmembrane-helix G protein-coupled receptors. They are involved as a modulator or transducer in various transmembrane signaling systems. G proteins are bound to GDP in the 'off' state. GNGT1 is the gamma subunit of transducin. Ligand-receptor binding results in detachment of the G protein, switching it to an 'on' state and permitting Galpha activation of second messenger signalling cascades. There are several types of Galpha proteins, in addition, some Gbetagamma subunits have active functions. Gbetagamma coupled to H1 receptors can activate PLA2 and Gbetagamma coupled to M1 receptors can activate KIR channels. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

Synonym: GNG1

Molecular Weight: 9.9kDa

UniProt: P63211

Pathways: Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Phototransduction