Intrinsic Factor Protein (His tag)
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- Target See all Intrinsic Factor (GIF) Proteins
- Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Intrinsic Factor protein is labelled with His tag.
- Purpose
- Recombinant Human Gastric intrinsic factor/GIF Protein (His Tag)
- Sequence
- Met 1-Tyr417
- Characteristics
- A DNA sequence encoding the human GIF (P27352-1) (Met1-Tyr417) was expressed with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product GIF Protein
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Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) (AA 318-417) protein (GST tag)
GIF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) (AA 1-417) protein (GST tag)GIF Origin: Human Host: Wheat germ Recombinant WB, ELISA, AA, AP
Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) (AA 19-417) protein (His tag)Crystallography grade GIF Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) (AA 19-417) protein (His tag)Crystallography grade GIF Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA, Crys
Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) protein (KLH)GIF Origin: Human Host: Synthetic Synthetic > 95 % WB, SDS, ELISA, IP
Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF) (AA 19-417) protein (His tag)GIF Origin: Human Host: Yeast Recombinant > 90 % ELISA
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Intrinsic Factor (GIF) (Gastric Intrinsic Factor (Vitamin B Synthesis) (GIF))
- Alternative Name
- Gastric intrinsic factor/GIF (GIF Products)
- Synonyms
- GIF Protein, IF Protein, IFMH Protein, INF Protein, TCN3 Protein, AV073125 Protein, gastric intrinsic factor Protein, GIF Protein, Gif Protein
- Background
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Background: Gastric intrinsic factor, also known as GIF, belongs to the of the cobalamin transport protein family. It is a glycoprotein produced by the parietal cells of the stomach. Gastric intrinsic factor plays a key role in the absorption of vitamin B12 on in the small intestine. Vitamin B12 bounds to haptocorrin after entry into the stomach. The resulting complex enters the duodenum, where pancreatic enzymes digest haptocorrin. In the less acidic environment of the small intestine, B12 can then bind to gastric intrinsic factor. This new complex travels to the ileum, where special epithelial cells endocytose them. Inside the cell, B12 dissociates once again and binds to another protein, transcobalamin II. The new complex can exit the epithelial cells to enter the liver.
Synonym: IF,IFMH,INF,TCN3
- Molecular Weight
- 44.8 kDa
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