GFPT1 Protein
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- Target See all GFPT1 Proteins
- GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- Escherichia coli (E. coli)
- Purpose
- Recombinant Human GFPT1/GFAT Protein
- Sequence
- Gln 332-Glu 699
- Characteristics
- The sequence corresponding to amino acids (Gln 332-Glu 699) of human GFAT (AAA58502.1) was expressed and purified with two amino acids (Gly & Pro) at the N-terminus.
- Purity
- > 97 % as determined by reducing SDS-PAGE.
- Top Product
- Discover our top product GFPT1 Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4, 10 % glycerol
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- GFPT1 (Glutamine--Fructose-6-Phosphate Transaminase 1 (GFPT1))
- Alternative Name
- GFPT1/GFAT (GFPT1 Products)
- Synonyms
- gfat Protein, 2810423A18Rik Protein, AI324119 Protein, AI449986 Protein, GFA Protein, GFAT Protein, GFAT1 Protein, GFAT1m Protein, Gfpt Protein, CMSTA1 Protein, GFAT 1 Protein, GFPT Protein, GFPT1L Protein, MSLG Protein, glutamine--fructose-6-phosphate transaminase 1 Protein, glutamine--fructose-6-phosphate transaminase 1 S homeolog Protein, glutamine fructose-6-phosphate transaminase 1 Protein, GFPT1 Protein, gfpt1.S Protein, Gfpt1 Protein
- Background
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Background: Glutamine:fructose-6-phosphate amidotransferase 1 (GFAT), also known as GFPT1, is a member of the N-terminal nucleophile aminotransferases and the first rate-limiting enzyme for the entry of glucose into the hexosamine biosynthesis pathway (HBP) in mammals. GFAT transfers the amino group from the L-glutamine amide to the D-fructose 6-phosphate, producing glutamic acid and glucosamine 6-phosphate. GFAT exists as a homotetramer in cytoplasm, and is proposed to be most likely involved in regulating the availability of precursors for N- and O-linked glycosylation of proteins. The full length of human GFAT contains 1 glutamine amidotransferase type-2 domain which catalyzes amide nitrogen transfer from glutamine to the appropriate substrate, and 2 SIS (Sugar Isomerase) domains found in many phosphosugar isomerases and phosphosugar binding proteins.Two isoforms of gfat have been identified: GFAT1 is predominantly expressed in skeletal muscle, whereas GFAT2 is expressed mainly in the central nervous system.
Synonym: CMSTA1,GFA,GFAT,GFAT1,GFAT1m,GFPT,GFPT1L,MSLG
- Molecular Weight
- 41.5 kDa
- Pathways
- ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process
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