Histone-Lysine N-Methyltransferase SMYD3 (SMYD3) protein (His tag,DYKDDDDK Tag)
-
- Target
- Histone-Lysine N-Methyltransferase SMYD3 (SMYD3)
- Protein Type
- Recombinant
- Origin
- Human
-
Source
- HEK-293 Cells
- Purification tag / Conjugate
- His tag,DYKDDDDK Tag
- Purpose
- Recombinant Human SMYD3/ZMYND1 Protein (His&FLAG Tag)
- Sequence
- Met 1-Ser 428
- Characteristics
- A DNA sequence encoding the human SMYD3 isoform 1 (NP_001161212.1) (Met 1-Ser 428) was fused with the flag tag at the N-terminus. and the Histidine tag at the C-terminus.
- Purity
- > 70 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
-
-
- Restrictions
- For Research Use only
-
- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 25 mM Tris-HCl, 100 mM NaCl, 20 % glycerol, 3 mM DTT, pH 8.0
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
-
- Target
- Histone-Lysine N-Methyltransferase SMYD3 (SMYD3)
- Alternative Name
- SMYD3/ZMYND1
- Background
-
Background: SET and MYND domain-containing protein 3, also known as Zinc finger MYND domain-containing protein 1, SMYD3, and ZMYND, is a member of the histone-lysine methyltransferase family. SMYD3 contains one MYND-type zinc finger and one SET domain. SMYD3 is a histone H3 lysine-4-specific methyltransferase. It is expressed in skeletal muscles and testis. It is overexpressed in a majority of colorectal carcinoma (CRC) and hepatocellular carcinoma (HCC). SMYD3 plays an important role in transcriptional regulation in human carcinogenesis. It activates the transcription of a set of downstream genes. Of these downstream genes, there are several oncogenes and genes associated with cell adhesion (including those of N-Myc, CrkL, Wnt10b, L-selectin, CD31 and galectin-4), which have been shown to have effects on cell viability, adhesion, migration and metastasis. Increased SMYD3 expression is essential for the proliferation of breast cancer cells. SMYD3 may be a promising new target of therapeutic intervention for the treatment of cancers or other pathological processes associated with cell adhesion and migration.
Synonym: bA74P14.1;KMT3E;ZMYND1;ZNFN3A1
- Molecular Weight
- 51.5 kDa
- NCBI Accession
- NP_001161212
-
