HAPLN1 Protein (His tag)

Catalog No. ABIN7317915

Product Details

Target: HAPLN1 (Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This HAPLN1 protein is labelled with His tag.

Purpose: Recombinant Human HAPLN1 Protein (His Tag)

Sequence: Met 1-Asn 354

Characteristics: A DNA sequence encoding the human HAPLN1 (NP_001875.1) (Met 1-Asn 354) was expressed with a C-terminal polyhistidine tag.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: HAPLN1 (Hyaluronan and Proteoglycan Link Protein 1 (HAPLN1))

Alternative Name: HAPLN1 (HAPLN1 Products)

Synonyms: crtl1 Protein, hapln1 Protein, wu:fc26f11 Protein, wu:fc37g04 Protein, wu:fc50e12 Protein, wu:fc55h01 Protein, CRTL1 Protein, LP Protein, BB099155 Protein, CLP Protein, Crtl1 Protein, Crtl1l Protein, LP-1 Protein, CTRL1 Protein, hyaluronan and proteoglycan link protein 1 Protein, hyaluronan and proteoglycan link protein 1a Protein, HAPLN1 Protein, hapln1a Protein, Hapln1 Protein

Background:

Background: Hyaluronan (HA) is a high MW glycosaminoglycan significantly involved in the formation and stability of extracellular matrix via its association with specific HA-binding proteins. HAPLN1, also known as CRTL1 (Cartilage Link Protein 1, cLP ) and link protein, is a member of HA-binding protein (hyaladherins) family, and contains a common structural domain of about 100 amino acids that is termed a Link module with two α-helices and two antiparallel β-sheets. HAPLN1/CRTL1 stabilizes the interaction between hyaluronan (HA) and versican, two extracellular matrix components essential for cardiac development. Link module superfamily can be divided into three subgroups, and the HAPLN family are C domain-type proteins that have an extended structure with one N-terminal V-type Ig-like domain followed by two link modules. In cartilage, aggrecan forms - cLP stabilized aggregates with HA that provides the tissue with its load bearing properties. HAPLN1 is a component of follicular matrix, was shown to enhance cumulus-oocyte complex (COC) expansion in vitro. HAPLN1 may promote periovulatory granulosa cell survival, which would facilitate their differentiation into luteal cells.

Synonym: CRTL1

Molecular Weight: 40 kDa

NCBI Accession: NP_001875