Insulin Protein (INS) (AA 25-53, AA 90-110)

Catalog No. ABIN7318503

Recombinant Insulin protein expressed in Yeast.

Quick Overview for Insulin Protein (INS) (AA 25-53, AA 90-110) (ABIN7318503)

Target: Insulin (INS)

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Yeast

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 25-53, AA 90-110

Purpose: Recombinant Human Insulin Protein

Sequence: Phe25-Lys53&Gly90-Asn110

Characteristics: A DNA sequence encoding human insulin B chain (2LGB_B)(Phe1-Lys29) and human insulin A chain (2KXK_A)(Gly1-Asn21) was expressed with a linker AAK.

Sterility: 0.2 μm filtered

Biological Activity Comment: Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED₅₀ for this effect is typically 0.2-1 μg/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Insulin (INS)

Alternative Name: Insulin

Background: IDDM,IDDM1,IDDM2,ILPR,INS,IRDN,MODY10,After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into three peptides: the B chain and A chain peptides, which are covalently linked via two disulfide bonds to form insulin, and C-peptide. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake.

Molecular Weight:

Calculated MW: 7.1 kDa

Observed MW: 6 kDa

Gene ID: 3630

UniProt: P01308

Pathways: NF-kappaB Signaling, RTK Signaling, Positive Regulation of Peptide Hormone Secretion, Peptide Hormone Metabolism, Hormone Activity, Carbohydrate Homeostasis, ER-Nucleus Signaling, Regulation of Carbohydrate Metabolic Process, Feeding Behaviour, Autophagy, Negative Regulation of intrinsic apoptotic Signaling, Brown Fat Cell Differentiation, Positive Regulation of fat Cell Differentiation