IGFBP6 Protein (His tag)

Catalog No. ABIN7196219

Product Details

Target: IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This IGFBP6 protein is labelled with His tag.

Purpose: Recombinant Mouse IGFBP6/IBP-6 Protein (His Tag)(Active)

Sequence: Met 1-Gly 238

Characteristics: A DNA sequence encoding the mouse IGFBP6 (NP_032370.2) (Met 1-Gly 238) was expressed, with a polyhistidine tag at the C-terminus.

Purity: > 92 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit the biological activity of IGFII on MCF7 human breast adenocarcinoma cells (Karey, K.P. et al. (1988) Cancer Research 48:4083.). The ED50 for this effect is typically 1-4 μg/ml in the presence of 20 ng/mL mouse IGFII.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IGFBP6 (Insulin-Like Growth Factor Binding Protein 6 (IGFBP6))

Alternative Name: IGFBP6/IBP-6 (IGFBP6 Products)

Synonyms: IBP6 Protein, IGFBP-6 Protein, IGF-BP6 Protein, RBP6A Protein, IGFBP6 Protein, igfbp6 Protein, insulin like growth factor binding protein 6 Protein, insulin-like growth factor binding protein 6 Protein, IGFBP6 Protein, Igfbp6 Protein, IGFBP-6 Protein, igfbp6 Protein

Background:

Background: Insulin-like growth factor binding protein 6 (IGFBP6) is a 24- kDa protein that binds insulin-like growth factor 1 (IGF-1) and IGF-2 with high affinity and inhibits IGF action in vitro. The Insulin-like growth factor-binding protein also known as IGFBP serves as a carrier protein for Insulin-like growth factor 1. IGFBPs are clearly distinct but are sharing regions with strong homology. All members of the IGFBP family bind IGF-I and IGF-II with about equal affinity. Insulin-like growth factor (IGF) binding proteins (IGFBPs) have been shown to either inhibit or enhance the action of IGF, or act in an IGF-independent manner in the prostate. IGF-binding protein-4 (IGFBP-4) inhibits IGF-I action in vitro and is the most abundant IGFBP in the rodent arterial wall. IGFBP6 is directly downregulated by the beta-catenin/TCF complex in desmoid tumors, and imply a role for the IGF axis in the proliferation of desmoid tumors. There is mounting evidence that the structure of the IGFBP proteins plays a key role in the regulation of IGF bioavailability, by modulating its molecular size, capillary membrane permeability, target tissue specificity, cell membrane adherence and IGF affinity.

Synonym: IGFBP-6

Molecular Weight: 24 kDa

NCBI Accession: NP_032370

Pathways: WNT Signaling, Myometrial Relaxation and Contraction