ILKAP Protein (His tag)

Catalog No. ABIN7317343

Product Details

Target: ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This ILKAP protein is labelled with His tag.

Purpose: Recombinant Human ILKAP Protein (His Tag)

Sequence: Met 1-His 392

Characteristics: A DNA sequence encoding the human ILKAP (Q9H0C8) (Met 1-His 392) was expressed, with a C-terminal polyhistidine tag.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: ILKAP (Integrin-Linked Kinase-Associated Serine/threonine Phosphatase 2C (ILKAP))

Alternative Name: ILKAP (ILKAP Products)

Synonyms: MGC84595 Protein, MGC107938 Protein, ILKAP Protein, ILKAP2 Protein, ILKAP3 Protein, PP2C-DELTA Protein, 0710007A14Rik Protein, 1600009O09Rik Protein, ILK associated serine/threonine phosphatase Protein, ILK associated serine/threonine phosphatase L homeolog Protein, integrin-linked kinase-associated serine/threonine phosphatase Protein, integrin-linked kinase-associated serine/threonine phosphatase 2C Protein, ILKAP Protein, ilkap.L Protein, ilkap Protein, Ilkap Protein

Background:

Background: Integrin-linked kinase-associated serine/threonine phosphatase 2C, also known as ILKAP, is cytoplasm protein which belongs to the PP2C family. ILKAP contains one PP2C-like domain. ILKAP is widely expressed. Highest levels expressed in striated muscle. Much lower levels evident in various smooth muscle tissues. ILKAP may play a role in regulation of cell cycle progression via dephosphorylation of its substrates whose appropriate phosphorylation states might be crucial for cell proliferation. ILKAP selectively associates with integrin linked kinase (ILK), to modulate cell adhesion and growth factor signaling. ILKAP inhibits the ILK-GSK3B signaling axis and may play an important role in inhibiting oncogenic transformation. Integrin-linked kinase ( ILK ) plays key roles in a variety of cell functions, including cell proliferation, adhesion and migration. Within the cell, ILK localizes to multiple sites, including the cytoplasm, focal adhesion complexes that mediate cell adhesion to extracellular substrates, as well as cell-cell junctions in epidermal keratinocytes. Nuclear ILK can be rapidly exported into the cytoplasm through a CRM1-dependent pathway, and its export is enhanced by the type 2C protein phosphatase ILKAP. Nuclear localization of ILK in epidermal keratinocytes is associated with increased DNA synthesis, which is sensitive to inhibition by ILKAP.

Synonym: ILKAP2;ILKAP3;PP2C-DELTA

Molecular Weight: 44.3 kDa

UniProt: Q9H0C8