IFI30 Protein (His tag)

Catalog No. ABIN7317348

Product Details

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Protein Type: Recombinant

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This IFI30 protein is labelled with His tag.

Purpose: Recombinant Human IFI30 Protein (His Tag)

Sequence: Met 1-Lys243

Characteristics: A DNA sequence encoding the human IFI30 (P13284) (Met1-Lys243) with a C-terminal polyhistidine tag was expressed.

Purity: > 96 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: IFI30 (Interferon, gamma-Inducible Protein 30 (IFI30))

Alternative Name: IFI30 (IFI30 Products)

Synonyms: GILT Protein, wu:fb12b04 Protein, zgc:101752 Protein, IP30 Protein, IFI-30 Protein, interferon, gamma-inducible protein 30 Protein, IFI30, lysosomal thiol reductase Protein, interferon gamma inducible protein 30 Protein, ifi30 Protein, IFI30 Protein, LOC100304719 Protein, Ifi30 Protein

Background:

Background: IFI30 belongs to the GILT family. This family includes the two characterised human gamma-interferon-inducible lysosomal thiol reductase (GILT) sequences: P13284 and Q9UL08. It also contains several other eukaryotic putative proteins with similarity to GILT. The aligned region contains three conserved cysteine residues. In addition, the two GILT sequences possess a C-X(2)-C motif that is shared by some of the other sequences in the family. This motif is thought to be associated with disulphide bond reduction. IFI30 is a lysosomal thiol reductase that can reduce protein disulfide bonds. It facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. It also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation. IFI30 may facilitate the complete unfolding of proteins destined for lysosomal degradation and plays an important role in antigen processing.

Synonym: GILT,IFI-30,IFI30,IP-30,IP30

Molecular Weight: 24.7 kDa

UniProt: P13284