IL-8 Protein (AA 28-99) (His tag)

Catalog No. ABIN7318687

This Recombinant IL-8 protein is expressed in Escherichia coli (E. coli).

Quick Overview for IL-8 Protein (AA 28-99) (His tag) (ABIN7318687)

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 98 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 28-99

Purification tag / Conjugate: This IL-8 protein is labelled with His tag.

Purpose: Recombinant Human IL-8/CXCL8 Protein

Sequence: Ser28-Ser99

Characteristics: Recombinant Human Interleukin-8 is produced by our E.coli expression system and the target gene encoding Ser28-Ser99 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.1 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measure by its ability to chemoattract PBMC. The ED₅₀ for this effect is <2 ng/mL.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile PBS, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: IL-8 (IL8) (Interleukin 8 (IL8))

Alternative Name: IL-8/CXCL8

Background: CXCL,Interleukin,C-X-C motif chemokine,Chemokine (C-X-C motif) ligand,IL8/CXCL,GCP,NAP,CXCL8,GCP-1,GCP1,IL8,LECT,LUCT,LYNAP,MDNCF,MONAP,NAF,NAP-1,NAP1,3-10C,AMCF-I,IL8/CXCL8,K60,Interleukin-8,IL-8,Emoctakin,C-X-C motif chemokine 8,Chemokine (C-X-C motif) ligand 8,Granulocyte chemotactic protein 1 (GCP-1),Monocyte-derived neutrophil chemotactic factor (MDNCF),(Ala-IL-8)77,(Ser-IL-8)72,9E3,Beta thromboglobulin like protein,CEF-4,chemokine,CXC motif,GCP/IL-8 protein I,GCP/IL-8 protein II,GCP/IL-8 protein III,GCP/IL-8 protein IV,GCP/IL-8 protein V,GCP/IL-8 protein VI,Granulocyte chemotactic protein 1,IL-8(1-77),IL-8(9-77),IL8/NAP1 form I,IL8/NAP1 form II,IL8/NAP1 form III,IL8/NAP1 form IV,IL8/NAP1 form V,IL8/NAP1 form VI,Inteleukin 8,ligand 8,Lymphocyte-derived neutrophil-activating factor,MDNCF-b,MDNCF-c,Monocyte-derived neutrophil chemotactic factor,Monocyte-derived neutrophil-activating peptide,Neutrophil activating peptide 1,Neutrophil activating protein 1,Neutrophil-activating factor,Neutrophil-activating protein 1,Protein 3 10C,Protein 3-10C,SCYB8,Small inducible cytokine subfamily B member 8,T cell chemotactic factor,T-cell chemotactic factor,TSG1,Interleukin-8 (IL-8) belongs to the neutrophil-specific CXC family of chemokines. It is one of the initial cytokines released from a variety of cell types, including T cells, endothelial cells and fibroblasts, in response to an inflammatory stimulus and acts by recruiting neutrophils, T-cells and basophils to the site of inflammation. Elevated Interleukin-8 levels are associated with the onset of a variety of disease states.

Molecular Weight:

Calculated MW: 9.3 kDa

Observed MW: 11 kDa

Gene ID: 3576

UniProt: P10145

Pathways: TLR Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of G-Protein Coupled Receptor Protein Signaling, ER-Nucleus Signaling, Hepatitis C, Autophagy