Intestinal Alkaline Phosphatase Protein (ALPI) (His tag)
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- Target See all Intestinal Alkaline Phosphatase (ALPI) Proteins
- Intestinal Alkaline Phosphatase (ALPI)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This Intestinal Alkaline Phosphatase protein is labelled with His tag.
- Purpose
- Recombinant Human Alkaline Phosphatase/ALPI Protein (His Tag)(Active)
- Sequence
- Met 1-Asp 503
- Characteristics
- A DNA sequence encoding the mature form of human ALPI (P09923) (Met 1-Asp 503),without the pro peptide, was fused with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave a fluorogenic substrate, 4-Methylumbelliferyl phosphate (4-MUP).The specific activity is > 1,000 pmoles/min/μg.
- Top Product
- Discover our top product ALPI Protein
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- Intestinal Alkaline Phosphatase (ALPI)
- Alternative Name
- Alkaline Phosphatase/ALPI (ALPI Products)
- Synonyms
- IAP Protein, Alp1 Protein, S51097 Protein, 2010001C14Rik Protein, Akp6 Protein, gIAP Protein, alpi Protein, im:7149588 Protein, si:ch211-262h13.1 Protein, wu:fa95f04 Protein, zgc:110794 Protein, ALPI Protein, Alpi Protein, alkaline phosphatase, intestinal Protein, alkaline phosphatase, intestinal, tandem duplicate 1 Protein, intestinal-type alkaline phosphatase Protein, ALPI Protein, Alpi Protein, alpi.1 Protein, LOC100724954 Protein
- Background
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Background: ALPI encodes for intestinal phosphatase alkaline, a brush border metalloenzyme that hydrolyses phosphate from the lipid A moiety of lipopolysaccharides and thereby drastically reduces Toll-like receptor 4 agonist activity. ALPI mutations impaired either stability or catalytic activity of ALPI and rendered it unable to detoxify lipopolysaccharide-dependent signalling. ALPI mutations should be included in screening for monogenic causes of inflammatory bowel diseases and lay the groundwork for ALPI-based treatments in intestinal inflammatory disorders.
Synonym: IAP
- Molecular Weight
- 54 kDa
- UniProt
- P09923
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