LGALS8 Protein (GST tag)

Catalog No. ABIN7321180

Product Details

Target: LGALS8 (Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))

Protein Type: Recombinant

Biological Activity: Active

Origin: Rat

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This LGALS8 protein is labelled with GST tag.

Purpose: Recombinant Rat Galectin-8/LGALS8 Protein (GST Tag)(Active)

Sequence: Met 1-Trp 316

Characteristics: A DNA sequence encoding rat LGALS8 (NP_446314.2) (Met 1-Trp 316) was fused with the GST tag at the N-terminus.

Purity: > 90 % as determined by SDS-PAGE

Biological Activity Comment: Measured by its ability to agglutinate human red blood cells.The ED50 for this effect is typically 0.05-0.3 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, 10 % glycerol, 1 mM DTT, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: LGALS8 (Lectin, Galactoside-Binding, Soluble, 8 (LGALS8))

Alternative Name: Galectin-8/LGALS8 (LGALS8 Products)

Synonyms: LGALS8 Protein, 1200015E08Rik Protein, AI326142 Protein, D13Ertd524e Protein, Lgals-8 Protein, galectin-8 Protein, Gal-8 Protein, PCTA-1 Protein, PCTA1 Protein, Po66-CBP Protein, xgalectin-VIIIa Protein, galectin 8 Protein, lectin, galactose binding, soluble 8 Protein, lectin, galactoside binding soluble 8 S homeolog Protein, LGALS8 Protein, Lgals8 Protein, lgals8.S Protein

Background:

Background: The Galectin family of proteins, with specificity for Nacetyllactosaminecontaining glycoproteins, consists of beta-galactoside binding lectins containing homologous carbohydrate recognition domains (CRDs). They also possess hemagglutination activity, which is attributable to their bivalent carbohydrate binding properties. Galectins are active both intracellularly and extracellularly. Although they are localized primarily in the cytoplasm and lack a classical signal peptide, galectins can also be secreted by one or more unidentified, non-classical, secretory pathways. They have diverse effects on many cellular functions including adhesion, migration, polarity, chemotaxis, proliferation, apoptosis, and differentiation. Galectins may therefore play a key role in many pathological states, including autoimmune diseases, allergic reactions, inflammation, tumor cell metastasis, atherosclerosis, and diabetic complications. The galectins have been classified into the prototype galectins(1, 2, 5, 7, 10, 11, 13, 14), which contain one CRD and exist either as a monomer or a noncovalent homodimer. The chimera galectins(Galectin3) containing one CRD linked to a nonlectin domain, and the tandemrepeat Galectins(4, 6, 8, 9, 12) consisting of two CRDs joined by a linker peptide.Galectins lack a classical signal peptide and can be localized to the cytosolic compartments where they have intracellular functions. However, via one or more as yet unidentified nonclassical secretory pathways, galectins can also be secreted to function extracellularly. Individual members of the galectin family have different tissue distribution profiles and exhibit subtle differences in their carbohydrate-binding specificities. Each family member may preferentially bind to a unique subset of cell surface glycoproteins.

Synonym: 30 kDa S-type lectin,RL-30,gal-8,galectin-8,lectin,galactose binding,soluble 8

Molecular Weight: 63.2 kDa

NCBI Accession: NP_446314