LY86 Protein (His tag)

Catalog No. ABIN7320457

Product Details

Target: LY86 (Lymphocyte Antigen 86 (LY86))

Protein Type: Recombinant

Origin: Mouse

Source: HEK-293 Cells

Purification tag / Conjugate: This LY86 protein is labelled with His tag.

Purpose: Recombinant Mouse MD1 Protein (His Tag)

Sequence: Met 1-Ser 162

Characteristics: A DNA sequence encoding the extracellular domain (Met 1-Ser 162) of mouse LY86 (NP_034875.1) precursor was expressed with a C-terminal polyhistidine tag.

Purity: > 92 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: LY86 (Lymphocyte Antigen 86 (LY86))

Alternative Name: MD1 (LY86 Products)

Synonyms: MD-1 Protein, MMD-1 Protein, dJ80N2.1 Protein, MD1 Protein, LY86 Protein, lymphocyte antigen 86 Protein, LY86 Protein, Ly86 Protein

Background:

Background: MD-1 and MD-2 are secretory glycoproteins that exist on the cell surface in complexes with transmembrane proteins. MD-1 is anchored by radioprotective 105 (RP105) which is a molecule containing leucine-rich repeats and is expressed on B cells, dentritic cells and macrophages, while MD-2 is associated with TLR4. MD-1 is required for efficient RP105 cell surface expression and function. It is indicated that the RP105/MD1 complex, in conjunction with TLR4, mediates the innate immune response to LPS in B cells, and also plays a role in protecting against apoptosis, B-cell proliferation, etc. Mouse MD-1 cDNA encodes a 162 amino acid precursor protein with a putative 19 aa signal peptide and two potential N-linked glycosylation sites. It shares 40 % and 66 % amino acid sequence identity with chicken and human MD-1 respectively. MD-1 is mainly expressed in spleen, and also detectable in liver, brain, thymus, and kidney.

Synonym: MD-1,MD1

Molecular Weight: 18 kDa

NCBI Accession: NP_034875

Pathways: Toll-Like Receptors Cascades