LAG3 Protein (His tag)
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- Target See all LAG3 Proteins
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Cynomolgus
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Source
- Human Cells
- Purification tag / Conjugate
- This LAG3 protein is labelled with His tag.
- Purpose
- Recombinant Cynomolgus LAG-3/CD223 Protein (His Tag)
- Sequence
- Pro23-Leu450(Pro74)
- Characteristics
- Recombinant Cynomolgus LAG-3 is produced by our Mammalian expression system and the target gene encoding Pro23-Leu450(Pro74) is expressed with a 8His tag at the N-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Immobilized Cynomolgus LAG-3-His at 2μg/ml(100 μl/well) can bind Human FGL1-Fc. The ED50 of Cynomolgus LAG-3-His is 5.8 ug/ml .
- Top Product
- Discover our top product LAG3 Protein
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Lymphocyte-Activation Gene 3 (LAG3) (AA 23-434) protein (His tag)
LAG3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lymphocyte-Activation Gene 3 (LAG3) (AA 23-450) protein (Fc Tag)LAG3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE
Lymphocyte-Activation Gene 3 (LAG3) (AA 23-434) protein (His-Avi Tag,Biotin)LAG3 Origin: Human Host: HEK-293 Cells Recombinant > 95 % as determined by Tris-Bis PAGE,> 95 % as determined by HPLC
Lymphocyte-Activation Gene 3 (LAG3) protein (His tag)LAG3 Origin: Mouse Host: HEK-293 Cells Recombinant > 95% as determined by Tris-Bis PAGE
Lymphocyte-Activation Gene 3 (LAG3) (AA 23-450) (Active) protein (His tag)LAG3 Origin: Human Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Lymphocyte-Activation Gene 3 (LAG3) (AA 18-449) (Active) protein (Fc Tag)LAG3 Origin: Cynomolgus Host: HEK-293 Cells Recombinant >90 % as determined by SDS-PAGE. Active
Lymphocyte-Activation Gene 3 (LAG3) (AA 23-222) protein (His-IF2DI Tag)LAG3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % by SDS-PAGE gel analyses WB, ELISA
Lymphocyte-Activation Gene 3 (LAG3) (Active) protein (His tag)LAG3 Origin: Mouse Host: Human Cells Recombinant > 95 % as determined by SDS-PAGE Active
Lymphocyte-Activation Gene 3 (LAG3) protein (Fc Tag)LAG3 Origin: Cynomolgus Host: Human Cells Recombinant > 90 % as determined by reducing SDS-PAGE.
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from a 0.2 μm filtered solution of PBS,PH7.4.
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- LAG3 (Lymphocyte-Activation Gene 3 (LAG3))
- Alternative Name
- LAG-3/CD223 (LAG3 Products)
- Synonyms
- CD223 Protein, LAG-3 Protein, Ly66 Protein, lymphocyte activating 3 Protein, lymphocyte-activation gene 3 Protein, LAG3 Protein, Lag3 Protein
- Background
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Background: Lymphocyte Activating 3(LAG3) belongs to immunoglobulin (Ig) superfamily, Macaca fascicularis LAG3 cDNA encodes 533 amino acids (aa) that include a 17 aa signal sequence, a 451 aa extracellular domain (ECD) with two Ig-like domains, a transmembrane region and a highly charged cytoplasmic region. LAG3',s main ligand is MHC class II, to which it binds with higher affinity than CD4. The protein negatively regulates cellular proliferation, activation, and homeostasis of T cells, in a similar fashion to CTLA-4 and PD-1 and has been reported to play a role in Treg suppressive function.
Synonym: Lymphocyte Activating 3, LAG3, LAG-3, CD223
- Molecular Weight
- 47.3 kDa
- NCBI Accession
- XP_005570011
- Pathways
- Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints
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