LAG3 Protein (His tag)

Catalog No. ABIN7320610

Product Details

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Human Cells

Purification tag / Conjugate: This LAG3 protein is labelled with His tag.

Purpose: Recombinant Mouse LAG3 Protein (His Tag)(Active)

Sequence: Ser23-Leu442

Characteristics: Recombinant Mouse Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Ser23-Leu442 is expressed with a 6His tag at the C-terminus.

Purity: > 95 % as determined by SDS-PAGE

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Immobilized Mouse LAG-3-His at 10μg/ml(100 μl/well) can bind Biotinylated Human FGL1-Avi. The ED50 of Mouse LAG-3-His is 9.4 ug/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: LAG3 (Lymphocyte-Activation Gene 3 (LAG3))

Alternative Name: LAG3 (LAG3 Products)

Synonyms: CD223 Protein, LAG-3 Protein, Ly66 Protein, lymphocyte activating 3 Protein, lymphocyte-activation gene 3 Protein, LAG3 Protein, Lag3 Protein

Background:

Background: Lymphocyte-activation gene 3 (LAG3), also known as CD223, is a type I transmembrane protein with four extracellular Ig-like domains, designated D1 to D4 and belongs to the immunoglobulin superfamily. The gene for LAG3 lies adjacent to the gene for CD4 on human chromosome 12p13.32 and shares approximately 20 % identical to the CD4 gene. LAG3 is expressed on activated T cells, natural killer cells, B cells and plasmacytoid dendritic cells. LAG3 binds with high affinity to MHC class II molecules, and it interferes competitively with the binding of CD4 to MHC class II and thereby blocks the transduction of stimulatory signals mediated by this interaction. LAG3 negatively regulates cellular proliferation, activation, and homeostasis of T cells, and plays an important role in Treg suppressive function. LAG3 is the target of various drug development programs to develop new treatments for cancer and autoimmune disorders. The soluble form, sLAG-3, is being developed as a cancer drug.

Synonym: Lymphocyte activation gene 3 protein,CD223,Lag3

Molecular Weight: 46.2 kDa

UniProt: Q61790

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Cancer Immune Checkpoints