MEP1A Protein (His tag)
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- Target See all MEP1A Proteins
- MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
- Protein Type
- Recombinant
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This MEP1A protein is labelled with His tag.
- Purpose
- Recombinant Human MEP1A/PPHA Protein (His Tag)
- Sequence
- Met 1-Gln 601
- Characteristics
- A DNA sequence encoding the human MEP1A (NP_005579.2) extracellular domain (Met 1-Gln 601) was expressed, fused with a polyhistidine tag at the C-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Top Product
- Discover our top product MEP1A Protein
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Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (Active) protein (His tag)
MEP1A Origin: Mouse Host: HEK-293 Cells Recombinant > 95 % as determined by SDS-PAGE Active
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 66-712) protein (His tag)Crystallography grade MEP1A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 65-713) protein (His tag)Crystallography grade MEP1A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 66-746) protein (rho-1D4 tag)Crystallography grade MEP1A Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A) (AA 65-747) protein (rho-1D4 tag)Crystallography grade MEP1A Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. SDS, WB, ELISA, Crys
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- MEP1A (Meprin A, alpha (PABA Peptide Hydrolase) (MEP1A))
- Alternative Name
- MEP1A/PPHA (MEP1A Products)
- Synonyms
- AI098089 Protein, AW107200 Protein, Mep-1 Protein, Mep-1a Protein, Mep1 Protein, PPHA Protein, meprin A subunit alpha Protein, meprin 1 alpha Protein, Mep1a Protein, MEP1A Protein
- Background
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Background: Meprin A subunit alpha, also known as MEP1A, and Endopeptidase-2, is a single-pass type I membrane protein which belongs to the peptidase M12A family. MEP1A contains one EGF-like domain, one MAM domain, and one MATH domain. Meprins are unique plasma membrane and secreted metalloproteinases that are highly regulated at the transcriptional and post-translational levels. Meprin alpha and beta subunits are abundantly expressed in kidney and intestinal epithelial cells, are secreted into the urinary tract and intestinal lumen, and are found in leukocytes and cancer cells under certain conditions. Meprins are capable of proteolytically degrading extracellular matrix proteins, proteolytically processing bioactive proteins, and play a role in inflammatory processes. Meprin A and B are highly regulated, secreted and cell-surface homo- and hetero-oligomeric enzymes. Meprins are abundantly expressed in kidney and intestine. The multidomain alpha and beta subunits have high sequence identity. They have very different substrate specificities, oligomerization potentials and are differentially regulated. Meprin A appears to be an important therapeutic target and urinary excretion appears to be a potential biomarker of acute kidney injury ( AKI ).
Synonym: PPHA
- Molecular Weight
- 67.7 kDa
- NCBI Accession
- NP_005579
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