c-MET Protein (AA 956-1390) (His-GST)

Catalog No. ABIN7317093

This Recombinant c-MET protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for c-MET Protein (AA 956-1390) (His-GST) (ABIN7317093)

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Application: ELISA

Purity: > 90 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 956-1390

Purification tag / Conjugate: This c-MET protein is labelled with His-GST.

Purpose: Recombinant Human c-MET/HGFR Protein (aa 956-1390, His & GST Tag)

Sequence: Lys956-Ser1390

Characteristics: A DNA sequence encoding the human MET (P08581-1) (Lys956-Ser1390) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: 1. The specific activity was determined to be 10 nmol/min/mg using MBP as substrate. 2. Immobilized human HGFR (aa 956-1390) at 10 μg/ml (100 μl/well) can bind biotinylated human HGF-his with a linear range of 15.6-125 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 7.4, 10 % glycerol, 3 mM DTT

Preservative: Dithiothreitol (DTT)

Precaution of Use: This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: c-MET (MET) (Met Proto-Oncogene (MET))

Alternative Name: c-MET/HGFR

Background: AUTS9,DFNB97,HGFR,RCCP2,c-Met,Hepatocyte growth factor receptor (HGFR), also known as c-Met or mesenchymal-epithelial transition factor (MET), is a receptor tyrosine kinase (RTK) that has been shown to be overexpressed and/or mutated in a variety of malignancies. HGFR protein is produced as a single-chain precursor, and HGF is the only known ligand. Normal HGF/HGFR signaling is essential for embryonic development, tissue repair or wound healing, whereas aberrantly active HGFR has been strongly implicated in tumorigenesis, particularly in the development of invasive and metastatic phenotypes. HGFR protein is a multifaceted regulator of growth, motility, and invasion, and is normally expressed by cells of epithelial origin. Preclinical studies suggest that targeting aberrant HGFR signaling could be an attractive therapy in cancer.

Molecular Weight:

Calculated MW: 76.8 kDa

Observed MW: 68 kDa

Gene ID: 4233

UniProt: P08581

Pathways: RTK Signaling, Carbohydrate Homeostasis, Synaptic Membrane, Signaling of Hepatocyte Growth Factor Receptor