MAP1D Protein (His tag)

Catalog No. ABIN7317733

Product Details

Target: MAP1D (METAP1D) (Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MAP1D protein is labelled with His tag.

Purpose: Recombinant Human MAP1D Protein (His Tag)(Active)

Sequence: Arg 44-Ala 335

Characteristics: A DNA sequence encoding the human MAP1D (NP_954697.1) (Arg 44-Ala 335) was expressed, with an initial Met at the N-terminus and a polyhistidine tag at the C-terminus.

Purity: > 92 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measure by its ability to remove methionine from a fluorogenic peptide substrate H-Met-Gly-Pro-AMC, R&D Systems, Catalog#ES017. The resulting GP-AMC is cleaved by human DPPIV/CD26, R&D Systems, Catalog#1180SE. The specific activity is >30 pmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: MAP1D (METAP1D) (Methionyl Aminopeptidase Type 1D (Mitochondrial) (METAP1D))

Alternative Name: MAP1D (METAP1D Products)

Synonyms: MAP 1D Protein, MAP1D Protein, MetAP 1D Protein, Metap1l Protein, 2310066F24Rik Protein, 3110033D18Rik Protein, AV117938 Protein, Map1d Protein, Metapl1 Protein, map1d Protein, zgc:110461 Protein, methionyl aminopeptidase type 1D, mitochondrial Protein, methionyl aminopeptidase type 1D (mitochondrial) Protein, METAP1D Protein, Metap1d Protein, metap1d Protein

Background:

Background: Methionine aminopeptidase 1D, also known as MAP1D, is a member of the peptidase M24A family. N-terminal methionine removal is an important cellular process required for proper biological activity, subcellular localization, and eventual degradation of many proteins. The enzymes that catalyze this reaction are called Methionine aminopeptidases (MAPs). MAP1D is overexpressed in colon cancer cell lines and colon tumors as compared to normal tissues (at protein level). Downregulation of MAP1D expression by shRNA in HCT-116 colon carcinoma cells reduces anchorage-independant growth in soft agar. MAP1D binds two cobalt ions per subunit. The true nature of the physiological cofactor is under debate. MAP1D is also active with zinc, manganese or divalent ions. MAP1D removes the amino-terminal methionine from nascent proteins. It may also play an important role in colon tumorigenesis.

Synonym: Methionine Aminopeptidase 1D Mitochondrial; Methionyl Aminopeptidase Type 1D Mitochondrial; METAP1D; MAP1D;MAP 1D;MetAP 1D;Metap1l

Molecular Weight: 33.4 kDa

NCBI Accession: NP_954697