MAP2K6 Protein (His-GST)

Catalog No. ABIN7317108

Recombinant MAP2K6 protein expressed in Baculovirus infected Insect Cells.

Quick Overview for MAP2K6 Protein (His-GST) (ABIN7317108)

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Baculovirus infected Insect Cells

Purity: > 88 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This MAP2K6 protein is labelled with His-GST.

Purpose: Recombinant Human MKK6 Protein (207 Ser/Asp, 211 Thr/Asp, His & GST Tag)

Sequence: Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp

Characteristics: A DNA sequence encoding the human MAP2K6 isoform 1 (P52564-1) (Met 1-Asp 334, Ser 207/Asp, Thr 211/Asp) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: The specific activity was determined to be 1250 nmol/min/mg using inactive MAPK14 as substrate.

Application Details

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: Supplied as sterile solution of 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Expiry Date: 6 months

Target Details

Target: MAP2K6 (Mitogen-Activated Protein Kinase Kinase 6 (MAP2K6))

Alternative Name: MKK6

Background: MAP2K6 ,MAPKK6,MEK6,MKK6,PRKMK6,SAPKK-3,SAPKK3,Dual specificity mitogen-activated protein kinase kinase 6, also known as MAP kinase kinase 6, MAPKK 6, MAPK / ERK kinase 6, SAPKK3, MAP2K6, and MKK6, is a protein that belongs to the protein kinase superfamily, STE Ser / Thr protein kinase family and MAP kinase kinase subfamily. MAP2K6 / MKK6 contains one protein kinase domain. Mitogen-activated protein kinases are members of a conserved cascade of kinases involved in many signal transduction pathways. They stimulate phosphorylation of transcription factors in response to extracellular signals such as growth factors, cytokines, ultraviolet light, and stress-inducing agents. MAP2K6 / MKK6 exists in a variety of alternatively spliced isoforms with distinct patterns of tissue expression. Isoform 2 of MAP2K6 / MKK6 is only expressed in skeletal muscle. Isoform 1 of MAP2K6 / MKK6 is expressed in skeletal muscle, heart, and to a lesser extent in liver or pancreas.

Molecular Weight:

Calculated MW: 65.3 kDa

Observed MW: 60 kDa

Gene ID: 5608

UniProt: P52564

Pathways: MAPK Signaling, TLR Signaling, Activation of Innate immune Response, Regulation of Muscle Cell Differentiation, Toll-Like Receptors Cascades