MAP4K5 Protein (GST tag,His tag)

Catalog No. ABIN7317063

Product Details

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This MAP4K5 protein is labelled with GST tag,His tag.

Purpose: Recombinant Human MAP4K5/MEKKK5 Protein (His & GST Tag)

Sequence: Met 1-Tyr 846

Characteristics: A DNA sequence encoding the human MAP4K5 (NP_006566.2) (Met 1-Tyr 846) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 82 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Frozen, Liquid

Buffer: Supplied as sterile 50 mM Tris, 100 mM NaCl, 0.5 mM PMSF, 0.5 mM TCEP, pH 8.5

Storage: -20 °C

Storage Comment: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Target Details

Target: MAP4K5 (Mitogen-Activated Protein Kinase Kinase Kinase Kinase 5 (MAP4K5))

Alternative Name: MAP4K5/MEKKK5 (MAP4K5 Products)

Synonyms: GCKR Protein, KHS Protein, KHS1 Protein, MAPKKKK5 Protein, 4432415E19Rik Protein, RGD1562028 Protein, fl74c10 Protein, wu:fl74c10 Protein, zgc:55719 Protein, zgc:55985 Protein, mitogen-activated protein kinase kinase kinase kinase 5 Protein, mitogen-activated protein kinase kinase kinase kinase 5 S homeolog Protein, MAP4K5 Protein, Map4k5 Protein, map4k5 Protein, map4k5.S Protein

Background:

Background: Mitogen-activated protein kinase kinase kinase kinase 5, also known as Kinase homologous to SPS1/STE20, MAPK/ERK kinase kinase kinase 5, MEK kinase kinase 5 and MAP4K5, is a cytoplasm protein which belongs to the protein kinase superfamily, STE Ser/Thr protein kinase family and STE20 subfamily. MAP4K5 is ubiquitously expressed in all tissues examined, with high levels in the ovary, testis and prostate. It contains one CNH domain and one protein kinase domain. MAP4K5 is highly similar to yeast SPS1/STE20 kinase. Yeast SPS1/STE20 functions near the beginning of the MAP kinase signal cascades that is essential for yeast pheromone response. MAP4K5 has been shown to interact with CRKL and TRAF2. This kinase was shown to activate Jun kinase in mammalian cells. MAP4K5 is an early component of MAP kinase signal cascades. It may play a role in the response to environmental stress. MAP4K5 appears to act upstream of the JUN N-terminal pathway.

Synonym: GCKR;KHS;KHS1;MAPKKKK5

Molecular Weight: 123 kDa

NCBI Accession: NP_006566