N6AMT1 Protein (His tag)

Catalog No. ABIN7317384

Product Details

Target: N6AMT1 (N-6 Adenine-Specific DNA Methyltransferase 1 (Putative) (N6AMT1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This N6AMT1 protein is labelled with His tag.

Purpose: Recombinant Human N6AMT1/HEMK2 Protein (His Tag)

Sequence: Met 1-Ser 186

Characteristics: A DNA sequence encoding the mature form of human N6AMT1 (AAH11554.1) (Met 1-Ser 186) was fused with a polyhistidine tag at the C-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: N6AMT1 (N-6 Adenine-Specific DNA Methyltransferase 1 (Putative) (N6AMT1))

Alternative Name: N6AMT1/HEMK2 (N6AMT1 Products)

Synonyms: HEMK2 Protein, C21orf127 Protein, N6AMT1 Protein, MTQ2 Protein, N6AMT Protein, 5830445C04Rik Protein, Hemk2 Protein, Pred28 Protein, RGD1311843 Protein, N-6 adenine-specific DNA methyltransferase 1 (putative) Protein, N-6 adenine-specific DNA methyltransferase 1 Protein, HemK methyltransferase family member 2 Protein, uncharacterized LOC100283871 Protein, N6AMT1 Protein, CpipJ_CPIJ001152 Protein, LOC100283871 Protein, N6amt1 Protein

Background:

Background: Pyruvate dehydrogenase kinase, isozyme 1, also known as [Pyruvate dehydrogenase [lipoamide]] kinase isozyme 1, mitochondrial and PDK1, is a member of the PDK / BCKDK protein kinase family. PDK-1 is expressed predominantly in the heart. It contains one histidine kinase domain. Pyruvate dehydrogenase kinase (PDK) isoforms are molecular switches that downregulate the pyruvate dehydrogenase complex (PDC) by reversible phosphorylation in mitochondria. An inhibitory effect of lipoic acid on PDKs would result in less phosphorylation of E1 and hence increased PDC activity. At least two isoenzymic forms of pyruvate dehydrogenase kinase ( PDK-1 and PDK-2 ) may be involved in the regulation of enzymatic activity of mammalian pyruvate dehydrogenase complex by phosphorylation. PDK-3 appears to have the highest specific activity among the three isoenzymes. PDK-1 inhibits the mitochondrial pyruvate dehydrogenase complex by phosphorylation of the E1 alpha subunit, thus contributing to the regulation of glucose metabolism.

Synonym: C21orf127;HEMK2;m.HsaHemK2P;MTQ2;N6AMT;PRED28

Molecular Weight: 21.2 kDa