NCKIPSD Protein (GST tag)

Catalog No. ABIN7317220

Product Details

Target: NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NCKIPSD protein is labelled with GST tag.

Purpose: Recombinant Human NCKIPSD/SPIN90 Protein (GST Tag)

Sequence: Met 1-Thr 244

Characteristics: A DNA sequence encoding the mature form of human NCKIPSD (Q9NZQ3-3) (Met1-Thr244) was expressed with the GST tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NCKIPSD (NCK Interacting Protein with SH3 Domain (NCKIPSD))

Alternative Name: NCKIPSD/SPIN90 (NCKIPSD Products)

Synonyms: AF3P21 Protein, DIP Protein, DIP1 Protein, ORF1 Protein, SPIN90 Protein, VIP54 Protein, WASLBP Protein, WISH Protein, Wasbp Protein, SH3 adapter protein SPIN90 Protein, NCK interacting protein with SH3 domain Protein, CpipJ_CPIJ019280 Protein, NCKIPSD Protein, Nckipsd Protein

Background:

Background: NCKIPSD is localized exclusively in the cell nucleus. It plays a role in signal transduction, and may function in the maintenance of sarcomeres and in the assembly of myofibrils into sarcomeres. NCKIPSD also plays an important role in stress fiber formation. NCKIPSD gene is involved in therapy-related leukemia by a chromosomal translocation t(3,11)(p21,q23) that involves this gene and the myeloid/lymphoid leukemia gene. Alternative splicing occurs in this locus and two transcript variants encoding distinct isoforms have been identified. NCKIPSD is a SH3 domain protein. Fas ligand is a cytotoxic effector molecule of T and NK cells which is characterized by an intracellular N-terminal polyproline region that serves as a docking site for SH3 and WW domain proteins. Several previously described Fas ligand-interacting SH3 domain proteins turned out to be crucial for the regulation of storage, expression and function of the death factor. Recent observations, however, indicate that Fas ligand is also subject to posttranslational modifications including shedding and intramembrane proteolysis.

Synonym: AF3P21,DIP,DIP1,ORF1,SPIN90,VIP54,WASLBP,WISH

Molecular Weight: 53.2 kDa

Pathways: Protein targeting to Nucleus