Nemo-Like Kinase Protein (NLK) (GST tag,His tag)
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- Target See all Nemo-Like Kinase (NLK) Proteins
- Nemo-Like Kinase (NLK)
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This Nemo-Like Kinase protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human NLK Protein (His & GST Tag)(Active)
- Sequence
- Val121-Glu 527
- Characteristics
- A DNA sequence encoding the human NLK (Q9UBE8) (Val121-Glu527) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 91 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be 3 nmol/min/mg using MBP as substrate.
- Top Product
- Discover our top product NLK Protein
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Nemo-Like Kinase (NLK) (AA 1-515) protein (GST tag)
NLK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA, KAA
Nemo-Like Kinase (NLK) (AA 121-515) (Active) protein (GST tag)NLK Origin: Human Host: Insect cells (Sf21) Recombinant SDS, Func Active
Nemo-Like Kinase (NLK) (AA 1-515) (Active) protein (His-GST)NLK Origin: Human Host: Insect cells (Sf9) Recombinant SDS, Func Active
Nemo-Like Kinase (NLK) (AA 416-515) protein (GST tag)NLK Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Nemo-Like Kinase (NLK) (AA 1-527) protein (Strep Tag)Crystallography grade NLK Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Nemo-Like Kinase (NLK) (AA 1-527) protein (Strep Tag)Crystallography grade NLK Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, SDS
Nemo-Like Kinase (NLK) protein (Myc-DYKDDDDK Tag)NLK Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 20 mM Tris, 500 mM NaCl, pH 8.0, 10 % glycerol
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
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- Target
- Nemo-Like Kinase (NLK)
- Alternative Name
- NLK (NLK Products)
- Synonyms
- Amf Protein, Gpi1 Protein, AI194375 Protein, nlk Protein, nlkb Protein, zgc:111875 Protein, Xnlk Protein, nlk1 Protein, nlk2 Protein, NLK Protein, nemo-like Protein, RGD1561602 Protein, glucose-6-phosphate isomerase Protein, nemo like kinase Protein, nemo-like kinase, type 1 Protein, nemo-like kinase L homeolog Protein, Nemo-Like kinase Protein, nemo-like kinase Protein, Gpi Protein, NLK Protein, Nlk Protein, nlk1 Protein, nlk.L Protein, nlk Protein, nemo-like Protein
- Background
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Background: Nemo-like kinase contains 1 protein kinase domain and belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and MAP kinase subfamily. It also contains a TQE activation loop motif in which autophosphorylation of the threonine residue (Thr-298) is sufficient for kinase activation. As a serine/threonine-protein kinase, nemo-like kinase regulates a number of transcription factors with key roles in cell fate determination. It is a positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Nemo-like kinase also is a negative regulator of the canonical Wnt/beta-catenin signaling pathway.
Synonym: LAK1,NLK
- Molecular Weight
- 74.1 kDa
- UniProt
- Q9UBE8
- Pathways
- Ubiquitin Proteasome Pathway
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