NGFB Protein (AA 130-239)

Catalog No. ABIN7320689

This Recombinant NGFB protein is expressed in Escherichia coli (E. coli).

Quick Overview for NGFB Protein (AA 130-239) (ABIN7320689)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Escherichia coli (E. coli)

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 130-239

Purpose: Recombinant Mouse β-NGF/NGFB Protein

Sequence: Met130-Arg239

Characteristics: Recombinant Mouse beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Met130-Arg239 is expressed. 

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 68.52 ng/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 200 mM NaCl, pH 8.0.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: beta-NGF/NGFB

Background: Beta-NGF,Beta-Nerve Growth Factor,NGF,NGFB,NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). These proteins belong to the cysteine-knot family of growth factors that assume stable dimeric structures. Mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90 % homology at the amino acid level with human beta -NGF and 95.8 % with rat beta -NGF. NGF signaling has been shown to play an important role in neuroprotection and repair. β-NGF acts as a growth and differentiation factor for B lymphocytes, and enhances B-cell survival. It is a potent neurotrophic factor that signals through its receptor β-NGFR, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems.

Molecular Weight:

Calculated MW: 12.4 kDa

Observed MW: 13 kDa

Gene ID: 18049

UniProt: P01139

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size