NGFB Protein

Catalog No. ABIN7319221

This Recombinant NGFB protein is expressed in Escherichia coli (E. coli).

Quick Overview for NGFB Protein (ABIN7319221)

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: Escherichia coli (E. coli)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Purpose: Recombinant Human β-NGF/NGFB Protein (aa 122-241, E. coli)(Active)

Sequence: Ser122-Ala241

Characteristics: Recombinant Human beta-Nerve Growth Factor is produced by our E.coli expression system and the target gene encoding Ser122-Ala241 is expressed.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.03-0.3 ng/ml.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 250 mM NaCl, pH 7.0.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: beta-NGF/NGFB

Background:

Background: Human β-Nerve Growth Factor (β-NGF) was initially isolated in the mouse submandibular gland. It is composed of three non-covalently linked subunits α, β, and γ, it exhibits all the biological activities ascribed to NGF. It is structurally related to BDNF, NT-3 and NT-4 and belongs to the cysteine-knot family of growth factors that assume stable dimeric structures. Β-NGF is a neurotrophic factor that signals through its receptor β-NGF, and plays a crucial role in the development and preservation of the sensory and sympathetic nervous systems. Β-NGF also acts as a growth and differentiation factor for B lymphocytes and enhances B-cell survival. These results suggest that β-NGF is a pleiotropic cytokine, which in addition to its neurotropic activities may have an important role in the regulation of the immune system. Human β-NGF shares 90 % sequence similarity with mouse protein and shows cross-species reactivity.

Synonym: Beta-Nerve Growth Factor, Beta-NGF, NGF, NGFB

Molecular Weight: 13.4 kDa

UniProt: P01138

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size