NGFB Protein (His tag)

Catalog No. ABIN7520353

Product Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NGFB protein is labelled with His tag.

Purpose: Recombinant Human Beta-NGF/NGF/NGFB Protein

Sequence: EPHSESNVPA GHTIPQAHWT KLQHSLDTAL RRARSAPAAA IAARVAGQTR NITVDPRLFK KRRLRSPRVL FSTQPPREAA DTQDLDFEVG GAAPFNRTHR SKRSSSHPIF HRGEFSVCDS VSVWVGDKTT ATDIKGKEVM VLGEVNINNS VFKQYFFETK CRDPNPVDSG CRGIDSKHWN SYCTTTHTFV KALTMDGKQA AWRFIRIDTA CVCVLSRKAV RRA

Specificity: Glu19-Ala241

Purity: > 92 % by SDS-PAGE.

Sterility: 0.22 μm filtered

Endotoxin Level: <0.01EU/μg

Biological Activity Comment: Recombinant Human NGF stimulates cell proliferation of the TF-1 human erythroleukemic cells. The ED₅₀ for this effect is 2.2-8.6 ng/mL, corresponding to a specific activity of 1.16x10⁵-4.55x10⁵ units/mg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid votex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1 % BSA, 5 % HSA, 10 % FBS or 5 % Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Concentration: 0.43 mg/mL

Buffer: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Storage: -20 °C,-80 °C

Storage Comment: Store the lyophilized protein at -20°C to -80°C for 12 months.|After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.

Target Details

Target: NGFB (Nerve Growth Factor beta (NGFB))

Alternative Name: Beta-NGF/NGF/NGFB (NGFB Products)

Synonyms: NGFB Protein, ngfb Protein, ns:zft386 Protein, xx:zft386 Protein, zgc:109730 Protein, hsan5 Protein, beta-ngf Protein, Ngfb Protein, Beta-NGF Protein, HSAN5 Protein, beta-NGF Protein, nerve growth factor Protein, nerve growth factor b (beta polypeptide) Protein, beta-Nerve growth factor Protein, nerve growth factor (beta polypeptide) Protein, NGF Protein, ngfb Protein, FPV072 Protein, FPV076 Protein, ngf Protein, Ngf Protein

Background:

Description: NGF is a well-characterized neurotropic protein that plays a critical role in the development of sympathetic and some sensory neurons in the peripheral nervous system. In addition, NGF can also act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons. NGF has also been shown to have biological effects on non-neuronal tissues. NGF is mitogenic for a factor?dependent human erythroleukemic cell line, TF-1. NGF has been found to increase the number of mast cells in neonatal rats and to induce histamine release from peritoneal mast cells. NGF will enhance histamine release and strongly modulate the formation of lipid mediators by basophils in response to various stimuli. NGF will also induce the growth and differentiation of human B lymphocytes as well as suppress apoptosis of murine peritoneal neutrophils. These results, taken together, suggest that NGF is a pleiotropic cytokine which, in addition to its neurotropic activities, may have an important role in the regulation of the immune system.

Name: NGFB, HSAN5, Beta-NGF

Gene ID: 4803

UniProt: P01138

Pathways: NF-kappaB Signaling, RTK Signaling, Regulation of Cell Size