NRG1-beta 1 Protein (Fc Tag)

Catalog No. ABIN7197136

This Recombinant NRG1-beta 1 protein is expressed in HEK-293 Cells.

Quick Overview for NRG1-beta 1 Protein (Fc Tag) (ABIN7197136)

Target: NRG1-beta 1 (Neuregulin-1 beta 1 (NRG1-beta 1))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purity: > 86 % as determined by reducing SDS-PAGE.

Product Details

Purification tag / Conjugate: This NRG1-beta 1 protein is labelled with Fc Tag.

Purpose: Recombinant Human Neuregulin-1/NRG1--β1 Protein (EGF Domain, Fc Tag)(Active)

Sequence: Thr 176-Lys 246

Characteristics: A DNA sequence encoding the EGF-like domain (Thr 176-Lys 246) of human NRG1 isoform beta1 (Q02297-6) was fused with the Fc region of human IgG1 at the N-terminus.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: 1. Measured by its binding ability in a functional ELISA.2. Immobilized Rhesus ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.58 μg/mL.3. Immobilized human ErbB3 at 2 μg/mL (100 μl/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NRG1-beta 1 (Neuregulin-1 beta 1 (NRG1-beta 1))

Alternative Name: Neuregulin-1/NRG1--beta1

Background:

Background: Neuregulin 1 or NRG1 is one of four proteins in the neuregulin family that act on the EGFR family of receptors. This growth factor was originally identified as a 44-kD glycoprotein that interacts with the NEU / ERBB2 receptor tyrosine kinase to increase its phosphorylation on tyrosine residues. NRG1 is a trophic factor that has been implicated in neural development; neurotransmission; and synaptic plasticity. NRG1 has multiple isoforms that are generated by usage of different promoters and alternative splicing of a single gene. Neuregulin 1 (NRG1) is essential for the development and function of multiple organ systems; and its dysregulation has been linked to diseases such as cancer and schizophrenia. NRG1 is a schizophrenia candidate gene and plays an important role in brain development and neural function. Schizophrenia is a complex disorder; with etiology likely due to epistasis. 

Synonym: Pro-neuregulin-1;Neuregulin-1 beta 1;NRG1-beta 1;HRG1-beta 1; EGF;NRG1; GGF; HGL; HRGA; NDF; SMDF;

Molecular Weight: 36.7 kDa