NRP2 Protein (Fc Tag)

Catalog No. ABIN7317794

Product Details

Target: NRP2 (Neuropilin 2 (NRP2))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Purification tag / Conjugate: This NRP2 protein is labelled with Fc Tag.

Purpose: Recombinant Human Neuropilin-2/NRP2 Protein (Fc Tag)(Active)

Sequence: Met 1-Tyr855

Characteristics: A DNA sequence encoding the human NRP2 (NP_003863.2)(Met1-Tyr855) was expressed with the Fc region of human IgG1 at the C-terminus.

Purity: > 95 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Biological Activity Comment: Measured by its binding ability in a functional ELISA.2.Immobilized human VEGFC-His at 10μg/mL (100μL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5μg/mL.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 7.4

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NRP2 (Neuropilin 2 (NRP2))

Alternative Name: Neuropilin-2/NRP2 (NRP2 Products)

Synonyms: NP2 Protein, NPN2 Protein, PRO2714 Protein, VEGF165R2 Protein, 1110048P06Rik Protein, Np-2 Protein, Np2 Protein, Npn-2 Protein, Npn2 Protein, neuropilin-2 Protein, neuropilin 2 Protein, NRP2 Protein, Nrp2 Protein

Background:

Background: Neuropilin-2 (NRP-2) which is related to NRP-1, is a type I? transmembrane glycoprotein and has the structure characteristic with five main extracellular domains: two complement binding (CUB) domains, two coagulation factor V/VIII homology domains, and a MAM (meprin, tyrosine phosphatase domain) region. NRP-2 is a receptor capable of binding two disparate ligands, classIII semaphorins (SEMA) and vascular endothelial growth factors (VEGF), and thus regulates two diverse systems by activating cellular signaling pathways via interacting with other cell surface receptors such as VEGF receptors and plexins. NRP-2 is well known for its role in facilitating axonal guidance during the development of the neuronal system, and additionally, it is also expressed in vascular endothelial cells and lymphatic endothelium where it affects proliferation, migration, angiogenesis, as well as formation of small lymphatic vessels and capillaries. Recent study has identified NRP-2 as a polysialylated protein expressed in human dendritic cells and modulates DC-T cell Interactions. Nearly all tumor cells express neuropilins and NRP-2 is predominantly expressed in neuronal tumors and melanomas. Furthermore, it is suggested that as the specific ligand for NRP-2, SEMA 3F inhibits tumor angiogenesis and metastasis.

Synonym: Neuropilin-2,NP2,NPN2,PRO2714,VEGF165R2

Molecular Weight: 120.7 kDa

NCBI Accession: NP_003863

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals