NOG Protein (AA 28-232)

Catalog No. ABIN7319511

This Recombinant NOG protein is expressed in HEK-293 Cells.

Quick Overview for NOG Protein (AA 28-232) (ABIN7319511)

Target: NOG (Noggin (NOG))

Protein Type: Recombinant

Biological Activity: Active

Origin: Human

Source: HEK-293 Cells

Application: Cell Culture (CC)

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 28-232

Purpose: Recombinant Human Noggin/NOG Protein

Sequence: Gln28-Cys232

Characteristics: Recombinant Human Noggin is produced by our Mammalian expression system and the target gene encoding Gln28-Cys232 is expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 0.01 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to inhibit BMP-4 induced alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED₅₀ for this effect is 10-100ng/mL in the presence of 200 ng/mL of Recombinant Human BMP-4(#CR93).

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 500 mM NaCl, 2 mM EDTA, pH 7.4.
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: NOG (Noggin (NOG))

Alternative Name: Noggin/NOG

Background: NOG,Noggin,Noggin is a secreted homodimeric glycoprotein that is an antagonist of bone morphogenetic proteins (BMPs). Mature Human Noggin contains an N-terminal acidic region, a central basic heparin-binding segment and a C-terminal cysteine-knot structure. Noggin is very highly conserved among vertebrates, such that mature human Noggin shares 99 % , 99 %, 98 %, 97 % and 89 % aa sequence identity with mouse, rat bovine, equine and chicken Noggin, respectively. Secreted Noggin probably remains close to the cell surface due to its binding of heparin-containing proteoglycans. Noggin binds some BMPs such as BMP4 with high affinity and others such as BMP7 with lower affinity. It antagonizes BMP bioactivities by blocking epitopes on BMPs that are needed for binding to both type I and type II receptors. Noggin is expressed in defined areas of the adult central nervous system and peripheral tissues such as lung, skeletal muscle and skin. During culture of human embryonic stem cells (hESC) or neural stem cells under certain conditions, addition of Noggin to antagonize BMP activity may allow stem cells to proliferate while maintaining their undifferentiated state, or alternatively, to differentiate into dopaminergic neurons.

Molecular Weight:

Calculated MW: 23 kDa

Observed MW: 25-33 kDa

Gene ID: 9241

UniProt: Q13253

Pathways: Stem Cell Maintenance, Tube Formation