NPM1 Protein (His tag)

Catalog No. ABIN7318061

Product Details

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Protein Type: Recombinant

Origin: Human

Source: Escherichia coli (E. coli)

Purification tag / Conjugate: This NPM1 protein is labelled with His tag.

Purpose: Recombinant Human NPM1/Nucleophosmin Protein (His Tag)

Sequence: Met 9-Leu 158

Characteristics: A DNA sequence encoding the human NPM1 isoform 1 (P06748-1) N-terminal segment (Met 9-Leu 158) was expressed, with a polyhistide tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile PBS, pH 6.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: NPM1 (Nucleophosmin (Nucleolar phosphoprotein B23, Numatrin) (NPM1))

Alternative Name: NPM1/Nucleophosmin (NPM1 Products)

Synonyms: B23 Protein, NPM Protein, NO38 Protein, Npm Protein, B23NP Protein, npm4 Protein, npm Protein, CG7917 Protein, CRP1 Protein, Crp1 Protein, Dmel\\CG7917 Protein, dNLP Protein, dNLP-S Protein, p22 Protein, npm1 Protein, sb:cb487 Protein, nucleophosmin 1 Protein, nucleophosmin (nucleolar phosphoprotein B23, numatrin) L homeolog Protein, nucleophosmin 1b Protein, Nucleophosmin Protein, nucleophosmin Protein, nucleophosmin (nucleolar phosphoprotein B23, numatrin) Protein, Nucleoplasmin Protein, nucleophosmin 1a Protein, NPM1 Protein, Npm1 Protein, npm1.L Protein, npm1b Protein, npm Protein, Nlp Protein, npm1a Protein

Background:

Background: Nucleophosmin 1 (NPM1), also known as nucleolar phosphoprotein B23 or numatrin, is a member of the nucleoplasmin family. Nucleophosmin (NPM) is a nucleolar phosphoprotein that plays multiple roles in ribosome assembly and transport, cytoplasmic-nuclear trafficking, centrosome duplication and regulation of p53. The NPM1 gene is frequently involved in chromosomal translocation, mutation and deletion. Mutations of the NPM1 gene leading to the expression of a cytoplasmic mutant protein, NPMc+, are the most frequent genetic abnormalities found in acute myeloid leukemias. Acute myeloid leukemias (AML) with mutated NPM1 have distinct characteristics, including a significant association with a normal karyotype, involvement of different hematopoietic lineages, a specific gene-expression profile and clinically, a better response to induction therapy and a favorable prognosis. In addition, NPM1 is a crucial gene to consider in the context of the genetics and biology of cancer. NPM1 is frequently overexpressed, mutated, rearranged and deleted in human cancer. Traditionally regarded as a tumour marker and a putative proto-oncogene, it has now also been attributed with tumour-suppressor functions.

Synonym: B23,NPM

Molecular Weight: 17.8 kDa

Pathways: Ribonucleoprotein Complex Subunit Organization, Ribosome Assembly