PDE1B Protein (GST tag,His tag)

Catalog No. ABIN7317495

Product Details

Target: PDE1B (phosphodiesterase 1B, Calmodulin-Dependent (PDE1B))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This PDE1B protein is labelled with GST tag,His tag.

Purpose: Recombinant Human PDE1B Protein (His & GST Tag)

Sequence: Met 1-Asp 536

Characteristics: A DNA sequence encoding the human PDE1B long isoform (Q01064-1) (Met 1-Asp 536) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 94 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 50 mM Tris, 100 mM NaCl, pH 8.0

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PDE1B (phosphodiesterase 1B, Calmodulin-Dependent (PDE1B))

Alternative Name: PDE1B (PDE1B Products)

Synonyms: 63kDa Protein, Pde1b1 Protein, PDE1B1 Protein, PDES1B Protein, Aop2 Protein, phosphodiesterase 1B, Ca2+-calmodulin dependent Protein, phosphodiesterase 1B Protein, Pde1b Protein, PDE1B Protein

Background:

Background: Calcium/calmodulin-dependent 3',5'-cyclic nucleotide phosphodiesterase 1B, also known as Cam-PDE 1B and PDE1B, is a cytoplasm protein which belongs to the cyclic nucleotide phosphodiesterase family and PDE1 subfamily. Phosphodiesterase-10A (PDE10A), Phosphodiesterase-1B (PDE1B), Phosphodiesterase-4B (PDE4B), and Phosphodiesterase-4A (PDE4A) are important regulators of signal transduction in striatum due to their catalysis of cyclic AMP and cyclic GMP. PDE1B is highly expressed in the striatum. It binds two divalent metal cations per subunit. Site one of PDE1B may preferentially bind zinc ions, while site two of PDE1B has a preference for magnesium and/or manganese ions. PDE1B is a cyclic nucleotide phosphodiesterase with a dual-specificity for the second messengers cAMP and cGMP, which are key regulators of many important physiological processes. It has a preference for cGMP as a substrate.

Synonym: PDE1B1;PDES1B

Molecular Weight: 89.2 kDa

Pathways: Neurotrophin Signaling Pathway, cAMP Metabolic Process, G-protein mediated Events, Interaction of EGFR with phospholipase C-gamma