PDE9A Protein (GST tag,His tag)

Catalog No. ABIN7317496

Product Details

Target: PDE9A (phosphodiesterase 9A (PDE9A))

Protein Type: Recombinant

Origin: Human

Source: Baculovirus infected Insect Cells

Purification tag / Conjugate: This PDE9A protein is labelled with GST tag,His tag.

Purpose: Recombinant Human PDE9A Protein (His & GST Tag)

Sequence: Met 1-Ala 533

Characteristics: A DNA sequence encoding the full length of human PDE9A (O76083-2) (Met 1-Ala 533) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Purity: > 90 % as determined by reducing SDS-PAGE.

Endotoxin Level: < 1.0 EU per μg as determined by the LAL method.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Please refer to the printed manual for detailed information.

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 5 mM GSH, pH 7.4, 10 % gly

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Target Details

Target: PDE9A (phosphodiesterase 9A (PDE9A))

Alternative Name: PDE9A (PDE9A Products)

Synonyms: PDE9A Protein, MGC116558 Protein, Pde9a Protein, HSPDE9A2 Protein, PDE9A1 Protein, phosphodiesterase 9A Protein, phosphodiesterase 9A S homeolog Protein, high affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A Protein, PDE9A Protein, pde9a.S Protein, Pde9a Protein, LOC100549810 Protein, pde9a Protein

Background:

Background: High affinity cGMP-specific 3';5'-cyclic phosphodiesterase 9A; also known as PDE9A; is a member of the cyclic nucleotide phosphodiesterase family and PDE9 subfamily. PDE9A is expressed in all tissues examined (testis; brain; small intestine; skeletal muscle; heart; lung; thymus; spleen; placenta; kidney; liver; pancreas; ovary and prostate) except blood. Highest levels of PDE9A is in brain; heart; kidney; spleen; prostate and colon. Isoform PDE9A12 is found in prostate. PDE9A mRNA is widely distributed throughout the rat and mouse brain; with the highest expression observed in cerebellar Purkinje cells. PDE9A is the only cGMP-specific PDE with significant expression in the forebrain; and as such is likely to play an important role in NO-cGMP signaling. PDE9A is highly conserved between species and is widely distributed throughout the rodent brain. PDE9A is probably involved in maintenance of low cGMP levels in cells and might play an important role in a variety of brain functions involving cGMP-mediated signal transduction. PDE9A hydrolyzes the second messenger cGMP; which is a key regulator of many important physiological processes. PDE9A represents a novel drug target worthy of further study.

Synonym: HSPDE9A2

Molecular Weight: 89.5 kDa