PLA2G2E Protein (His tag)
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- Target See all PLA2G2E Proteins
- PLA2G2E (Phospholipase A2, Group IIE (PLA2G2E))
- Protein Type
- Recombinant
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Origin
- Mouse
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PLA2G2E protein is labelled with His tag.
- Purpose
- Recombinant Mouse PLA2G2E Protein (His Tag)
- Sequence
- Met 1-Cys 142
- Characteristics
- A DNA sequence encoding the mouse PLA2G2E (Q9QUL3) (Met 1-Cys 142) was expressed, with a C-terminal polyhistidine tag.
- Purity
- > 97 % as determined by SDS-PAGE
- Endotoxin Level
- < 1.0 EU per μg of the protein as determined by the LAL method.
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- Discover our top product PLA2G2E Protein
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Phospholipase A2, Group IIE (PLA2G2E) (AA 1-142) protein (GST tag)
PLA2G2E Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Phospholipase A2, Group IIE (PLA2G2E) (AA 20-142) protein (His tag)Crystallography grade PLA2G2E Origin: Human Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
Phospholipase A2, Group IIE (PLA2G2E) (AA 20-142) protein (His tag)Crystallography grade PLA2G2E Origin: Mouse Host: Insect Cells Recombinant >95 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. ELISA, WB, Crys, SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile PBS, 10 % glycerol, pH 7.4
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PLA2G2E (Phospholipase A2, Group IIE (PLA2G2E))
- Alternative Name
- PLA2G2E (PLA2G2E Products)
- Synonyms
- GIIE sPLA2 Protein, sPLA2-IIE Protein, phospholipase A2 group IIE Protein, phospholipase A2, group IIE Protein, PLA2G2E Protein, Pla2g2e Protein
- Background
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Background: Group IIE secretory phospholipase A2, also known as GIIE sPLA2, sPLA2-IIE, Phosphatidylcholine 2-acylhydrolase 2E and PLA2G2E is a secreted protein which belongs to the phospholipase A2 family. Mammalian secretory phospholipase A2s ( sPLA2s ) form a family of structurally related enzymes that are involved in a variety of physiological and pathological processes via the release of arachidonic acid from membrane phospholipids or the binding to specific membrane receptors. Phospholipases A2 / PLA2 are enzymes that release fatty acids from the second carbon group of glycerol. This particular phospholipase specifically recognizes the sn-2 acyl bond of phospholipids and catalytically hydrolyzes the bond releasing arachidonic acid and lysophospholipids. Phospholipases A2 / PLA2 are commonly found in mammalian tissues as well as insect and snake venom. Venom from both snakes and insects is largely composed of melittin, which is a stimulant of Phospholipases A2 / PLA2. Due to the increased presence and activity of Phospholipases A2 / PLA2 resulting from a snake or insect bite, arachidonic acid is released from the phospholipid membrane disproportionately. As a result, inflammation and pain occur at the site. PLA2G2E catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides. Has a preference for arachidonic-containing phospholipids.
Synonym: PLA2G2E
- Molecular Weight
- 15.5 kDa
- UniProt
- Q9QUL3
- Pathways
- Inositol Metabolic Process
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