Pleiotrophin Protein (PTN) (AA 1-168)

Catalog No. ABIN7197415

This Recombinant Pleiotrophin protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for Pleiotrophin Protein (PTN) (AA 1-168) (ABIN7197415)

Target: Pleiotrophin (PTN)

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Application: ELISA

Purity: > 95 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 1-168

Purpose: Recombinant Mouse Pleiotrophin/PTN/HB-GAM Protein

Sequence: Met1-Asp168

Characteristics: A DNA sequence encoding the mouse PTN (P63089) (Met1-Asp168) was expressed.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Immobilized mouse PTN at 10 μg/ml (100 μl/well) can bind rat SDC1-Fc, The EC₅₀ of rat SDC1-Fc is 0.4-1. 1 μg/ml.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 1M NaCl, pH 7.4
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: Pleiotrophin (PTN)

Alternative Name: Pleiotrophin/PTN/HB-GAM

Background: HARP,HB-GAM,HBBM,HBBN,HBGF-8,HBNF,Heparin-binding brain mitogen,Heparin-binding growth factor 8,OSF,OSF-1,Osf-1,Osf1,Osteoblast-specific factor 1,PTN,Pleiotrophin,HB-GAM belongs to the pleiotrophin family. During embryonic and early postnatal development, HB-GAM is expressed in the central and peripheral nervous system and also in several non-neural tissues, notably lung, kidney, gut and bone. While in the adult central nervous system, it is expressed in an activity-dependent manner in the hippocampus where it can suppress long term potentiation induction. HB-GAM has a low expression in other areas of the adult brain, but it can be induced by ischemic insults, or targeted neuronal damaged in the entorhinal cortex or in the substantia nigra pars compacta. It is structurally related to midkine and retinoic acid induced heparin-binding protein and has a high affinity for heparin. HB-GAM binds anaplastic lymphoma kinase (ALK) which induces MAPK pathway activation, an important step in the anti-apoptotic signaling of PTN and regulation of cell proliferation. It also functions as a secreted growth factor and induces neurite outgrowth and which is mitogenic for fibroblasts, epithelial, and endothelial cells.

Molecular Weight:

Calculated MW: 15.3 kDa

Observed MW: 20 kDa

UniProt: P63089

Pathways: RTK Signaling