PRSS3 Protein (His tag)
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- Target See all PRSS3 Proteins
- PRSS3 (Protease, serine, 3 (PRSS3))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- HEK-293 Cells
- Purification tag / Conjugate
- This PRSS3 protein is labelled with His tag.
- Purpose
- Recombinant Human Trypsin-3/PRSS3 Protein (His Tag)(Active)
- Sequence
- Met 1-Ser 247
- Characteristics
- A DNA sequence encoding the human PRSS3 isoform c (P35030-3) (Met 1-Ser 247) was expressed, with a polyhistidine tag at the C-terminus.
- Purity
- > 95 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to cleave the fluorogenic peptide substrate, Mca-RPKPVE-Nval-WRK(Dnp)-NH2 (AnaSpec, Catalog#27114). The specific activity is >4,000 pmoles/min/μg.(Activation description: The proenzyme needs to be activated by enteropeptidase for an activated form)
- Top Product
- Discover our top product PRSS3 Protein
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Protease, serine, 3 (PRSS3) (AA 81-304) protein (His tag)
PRSS3 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE ELISA, WB
Protease, serine, 3 (PRSS3) (AA 1-247) protein (GST tag)PRSS3 Origin: Human Host: Wheat germ Recombinant ELISA, WB, AP, AA
Protease, serine, 3 (PRSS3) (AA 81-303), (partial) protein (His tag)PRSS3 Origin: Human Host: Escherichia coli (E. coli) Recombinant > 90 % SDS
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 50 mM MES, 0.6M NaCl, pH 5.0
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PRSS3 (Protease, serine, 3 (PRSS3))
- Alternative Name
- Trypsin-3/PRSS3 (PRSS3 Products)
- Synonyms
- MTG Protein, PRSS4 Protein, T9 Protein, TRY3 Protein, TRY4 Protein, Try3 Protein, Tb Protein, prss3 Protein, protease, serine 3 Protein, trypsin-3 Protein, protease, serine, 3 Protein, trypsin III Protein, protease, serine 3 L homeolog Protein, PRSS3 Protein, CpipJ_CPIJ016105 Protein, Prss3 Protein, trp-iii Protein, prss3.L Protein
- Background
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Background: Trypsin-3, also known as Trypsin III, brain trypsinogen, Serine protease 3 and PRSS3, is a secreted protein which belongs to the peptidase S1 family. Trypsin-3 / PRSS3 is expressed is in pancreas and brain. It contains one peptidase S1 domain. Trypsin-3 / PRSS3 can degrade intrapancreatic trypsin inhibitors that protect against CP. Genetic variants that cause higher mesotrypsin activity might increase the risk for chronic pancreatitis (CP). A sustained imbalance of pancreatic proteases and their inhibitors seems to be important for the development of CP. The trypsin inhibitor-degrading activity qualified PRSS3 as a candidate for a novel CP susceptibility gene. Trypsin-3 / PRSS3 has been implicated as a putative tumor suppressor gene due to its loss of expression, which is correlated with promoter hypermethylation, in esophageal squamous cell carcinoma and gastric adenocarcinoma.
Synonym: MTG;PRSS4;RP11-176F3.3;T9;TRY3;TRY4
- Molecular Weight
- 26.6 kDa
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