PKD2 Protein (GST tag,His tag)
-
- Target See all PKD2 Proteins
- PKD2 (Protein Kinase D2 (PKD2))
- Protein Type
- Recombinant
- Biological Activity
- Active
-
Origin
- Human
-
Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PKD2 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human PRKD2/PKD2 Protein (His & GST Tag)(Active)
- Sequence
- Met 1-Leu 878
- Characteristics
- A DNA sequence encoding the amino acid sequence (Met 1-Leu 878) of human PRKD2 (NP_057541.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 82 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- The specific activity was determined to be >30 nmol/min/mg using synthetic CREBtide peptide (KRREILSRRPSYR) as substrate.
- Top Product
- Discover our top product PKD2 Protein
-
Protein Kinase D2 (PKD2) (AA 1-878) protein (GST tag)
PKD2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase D2 (PKD2) (AA 1-110) protein (GST tag)PKD2 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Kinase D2 (PKD2) (AA 1-878) protein (Strep Tag)Crystallography grade PKD2 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Kinase D2 (PKD2) (AA 1-875) protein (Strep Tag)Crystallography grade PKD2 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Kinase D2 (PKD2) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PKD2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Kinase D2 (PKD2) (Transcript Variant 2) protein (Myc-DYKDDDDK Tag)PKD2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
Protein Kinase D2 (PKD2) (Transcript Variant 3) protein (Myc-DYKDDDDK Tag)PKD2 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
-
- Restrictions
- For Research Use only
-
- Format
- Frozen, Liquid
- Buffer
- Supplied as sterile 50 mM Tris, 500 mM NaCl, 0.5 mM PMSF, 10 % glycerol, pH 8.0
- Storage
- -20 °C
- Storage Comment
- Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.
-
- Target
- PKD2 (Protein Kinase D2 (PKD2))
- Alternative Name
- PRKD2/PKD2 (PKD2 Products)
- Synonyms
- PKD2 Protein, nPKC-D2 Protein, AI325941 Protein, RGD1308054 Protein, PRKD2 Protein, protein kinase D2 Protein, serine/threonine-protein kinase D2 Protein, PRKD2 Protein, Prkd2 Protein, LOC100407181 Protein
- Background
-
Background: Serine/threonine-protein kinase D2, also known as PRKD2 and PKD2, is a cytoplasm and membrane protein which belongs to the protein kinase superfamily, CAMK Ser/Thr protein kinase family and PKD subfamily. PRKD2 / PKD2 is widely expressed. It contains one PH domain, two phorbol-ester/DAG-type zinc fingers and one protein kinase domain. PRKD2 / PKD2 is activated by DAG and phorbol esters. Phorbol-ester/DAG-type domains bind DAG, mediating translocation to membranes. Autophosphorylation of Ser-710 and phosphorylation of Ser-706 by PKC relieves auto-inhibition by the PH domain. PRKD2 / PKD2 converts transient diacylglycerol (DAG) signals into prolonged physiological effects, downstream of PKC. Involved in resistance to oxidative stress.
Synonym: HSPC187;nPKC-D2;PKD2
- Molecular Weight
- 124 kDa
- NCBI Accession
- NP_057541
- Pathways
- cAMP Metabolic Process, Maintenance of Protein Location, Negative Regulation of Transporter Activity
-
