PTPN12 Protein (GST tag,His tag)
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- Target See all PTPN12 Proteins
- PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))
- Protein Type
- Recombinant
- Biological Activity
- Active
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Origin
- Human
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Source
- Baculovirus infected Insect Cells
- Purification tag / Conjugate
- This PTPN12 protein is labelled with GST tag,His tag.
- Purpose
- Recombinant Human PTPN12 Protein (aa 1-355, His & GST Tag)(Active)
- Sequence
- Met 1-Gln355
- Characteristics
- A DNA sequence encoding the human PTPN12 (AAA36529.1) (Met1-Gln355) was expressed with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
- Purity
- > 90 % as determined by reducing SDS-PAGE.
- Endotoxin Level
- < 1.0 EU per μg as determined by the LAL method.
- Biological Activity Comment
- Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988-998 phosphopeptide substrate, R&D Systems, Catalog # ES006.The specific activity is > 15 μmoles/min/mg.
- Top Product
- Discover our top product PTPN12 Protein
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Protein Tyrosine Phosphatase G1 (PTPN12) (AA 2-300) protein (His tag)
PTPN12 Origin: Human Host: Escherichia coli (E. coli) Recombinant Greater than 95 % as determined by SDS-PAGE WB, ELISA
Protein Tyrosine Phosphatase G1 (PTPN12) (AA 1-780) protein (GST tag)PTPN12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Tyrosine Phosphatase G1 (PTPN12) (AA 682-779) protein (GST tag)PTPN12 Origin: Human Host: Wheat germ Recombinant WB, ELISA, AP, AA
Protein Tyrosine Phosphatase G1 (PTPN12) (AA 1-780) protein (Strep Tag)Crystallography grade PTPN12 Origin: Human Host: Tobacco (Nicotiana tabacum) Recombinant >80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Tyrosine Phosphatase G1 (PTPN12) (AA 1-775) protein (Strep Tag)Crystallography grade PTPN12 Origin: Mouse Host: Tobacco (Nicotiana tabacum) Recombinant ≥ 80 % as determined by SDS PAGE, Size Exclusion Chromatography and Western Blot. WB, SDS, ELISA
Protein Tyrosine Phosphatase G1 (PTPN12) (Transcript Variant 1) protein (Myc-DYKDDDDK Tag)PTPN12 Origin: Human Host: HEK-293 Cells Recombinant > 80 % as determined by SDS-PAGE and Coomassie blue staining AbP, STD
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- Restrictions
- For Research Use only
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- Format
- Lyophilized
- Reconstitution
- Please refer to the printed manual for detailed information.
- Buffer
- Lyophilized from sterile 20 mM Tris, 500 mM NaCl, 10 % glycerol, pH 8.0
- Storage
- 4 °C,-20 °C,-80 °C
- Storage Comment
- Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.
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- Target
- PTPN12 (Protein Tyrosine Phosphatase G1 (PTPN12))
- Alternative Name
- PTPN12 (PTPN12 Products)
- Synonyms
- PTP-PEST Protein, PTPG1 Protein, P19-PTP Protein, PTP-P19 Protein, Ptpg1 Protein, RKPTP Protein, protein tyrosine phosphatase, non-receptor type 12 Protein, PTPN12 Protein, Ptpn12 Protein
- Background
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Background: PTPN12 is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. PTPN12 contains a C-terminal PEST motif, which serves as a protein-protein interaction domain, and may be related to protein intracellular half-life. PTPN12 was found to bind and dephosphorylate the product of oncogene c-ABL, thus may play a role in oncogenesis. PTPN12 was shown to interact with, and dephosphorylate, various of cytoskeleton and cell adhesion molecules, such as p130 (Cas), CAKbeta/PTK2B, PSTPIP1, and paxillin, which suggested its regulatory roles in controlling cell shape and mobilit.
Synonym: PTP-PEST,PTPG1
- Molecular Weight
- 69.4 kDa
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