SHP1 Protein (AA 207-597) (His-GST)

Catalog No. ABIN7320120

This Recombinant SHP1 protein is expressed in Baculovirus infected Insect Cells.

Quick Overview for SHP1 Protein (AA 207-597) (His-GST) (ABIN7320120)

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Protein Type: Recombinant

Biological Activity: Active

Origin: Mouse

Source: Baculovirus infected Insect Cells

Purity: > 85 % as determined by reducing SDS-PAGE.

Product Details

Protein Characteristics: AA 207-597

Purification tag / Conjugate: This SHP1 protein is labelled with His-GST.

Purpose: Recombinant Mouse PTPN6/SH-PTP1 Protein (aa 207-597, His & GST Tag)

Sequence: Ala207-Lys597

Characteristics: A DNA sequence encoding the mouse PTPN6 (P29351-2) (Ala207-Lys597) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.

Sterility: 0.2 μm filtered

Endotoxin Level: < 1.0 EU per μg of the protein as determined by the LAL method.

Biological Activity Comment: Measured by its ability to dephosphorylate a phosphotyrosine residue in an EGF receptor 988998 phosphopeptide substrate, R&D Systems, Catalog # ES006. The specific activity is > 4 μmoles/min/μg.

Application Details

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized from sterile 20 mM Tris, 500 mM NaCl, pH 7.0, 10 % glycerol
Normally 5 % - 8 % trehalose, mannitol and 0.01 % Tween 80 are added as protectants before lyophilization.

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.

Expiry Date: 12 months

Target Details

Target: SHP1 (PTPN6) (Protein-tyrosine Phosphatase 1C (PTPN6))

Alternative Name: PTPN6/SH-PTP1

Background: 70Z-SHP,Hcph,PTPTY-42,Ptp1C,SH-PTP1,SHP-1,hcp,me,PTPN6 is an enzyme which belongs to the protein tyrosine phosphatase (PTP) family. PTPs are signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. N-terminal part of PTPN6 contains two tandem Src homolog (SH2) domains, which act as protein phospho-tyrosine binding domains, and mediate the interaction of PTPN6 with its substrates. PTPN6 is expressed primarily in hematopoietic cells, and functions as an important regulator of multiple signaling pathways in hematopoietic cells. It has been shown that PTPN6 interacts with, and dephosphorylate a wide spectrum of phospho-proteins involved in hematopoietic cell signaling.

Molecular Weight:

Calculated MW: 72.7 kDa

Observed MW: 63 kDa

UniProt: P29351

Pathways: JAK-STAT Signaling, TCR Signaling, TLR Signaling, Nuclear Receptor Transcription Pathway, Positive Regulation of Peptide Hormone Secretion, Steroid Hormone Mediated Signaling Pathway, Response to Growth Hormone Stimulus, Regulation of Leukocyte Mediated Immunity, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, BCR Signaling